Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols

Janet V. Warg; Travis Clement; Emily R. Cornwell; Angela Cruz; Rodman G. Getchell; Cem Giray; Andrew E. Goodwin; Geoffrey H. Groocock; Mohamed Faisal; Robert Kim; Gwenn E. Merry; Nicholas B.D. Phelps; Monica M. Reising; Isaac Standish; Yan Zhang; Kathy Toohey-Kurth

doi:10.3354/dao02758

Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols

Janet V. Warg, Travis Clement, Emily R. Cornwell, Angela Cruz, Rodman G. Getchell, Cem Giray, Andrew E. Goodwin, Geoffrey H. Groocock, Mohamed Faisal, Robert Kim, Gwenn E. Merry, Nicholas B.D. Phelps, Monica M. Reising, Isaac Standish, Yan Zhang, Kathy Toohey-Kurth

Fisheries, Wildlife, and Conservation Biology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Two real-time reverse transcription polymerase chain reaction (rRT-PCR) assays under consideration for deployment to multiple testing laboratories across the USA were evaluated for diagnostic sensitivity and specificity on tissue homogenates obtained from natural and experimental viral hemorrhagic septicemia (VHS)-infected fish. Estimates for diagnostic specificity using virus isolation as the reference method were similar between laboratories regardless of the assay. Diagnostic sensitivity estimates of 0.96 (95% CI: 0.95, 0.97) for Jonstrup et al. (2013)'s assay (J Fish Dis 36:9-23) exceeded the diagnostic sensitivity of 0.85 (95% CI: 0.83, 0.87) for Phelps et al. (2012)'s assay (J Aquat Anim Health 24:238-243). The Jonstrup rRT-PCR assay is robust as demonstrated by high sensitivity and specificity estimates across laboratories and can be used as a valuable tool for targeted surveillance and for testing of suspect VHSV samples.

Original language	English (US)
Pages (from-to)	15-22
Number of pages	8
Journal	Diseases of aquatic organisms
Volume	111
Issue number	1
DOIs	https://doi.org/10.3354/dao02758
State	Published - Aug 21 2014

Keywords

Diagnostic sensitivity
Diagnostic specificity
Real-time RT-PCR
Surveillance
VHSV

Access

10.3354/dao02758

OpenUrl availability

Full text

Cite this

Warg, J. V., Clement, T., Cornwell, E. R., Cruz, A., Getchell, R. G., Giray, C., Goodwin, A. E., Groocock, G. H., Faisal, M., Kim, R., Merry, G. E., Phelps, N. B. D., Reising, M. M., Standish, I., Zhang, Y., & Toohey-Kurth, K. (2014). Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols. Diseases of aquatic organisms, 111(1), 15-22. https://doi.org/10.3354/dao02758

Warg, JV, Clement, T, Cornwell, ER, Cruz, A, Getchell, RG, Giray, C, Goodwin, AE, Groocock, GH, Faisal, M, Kim, R, Merry, GE, Phelps, NBD, Reising, MM, Standish, I, Zhang, Y & Toohey-Kurth, K 2014, 'Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols', Diseases of aquatic organisms, vol. 111, no. 1, pp. 15-22. https://doi.org/10.3354/dao02758

@article{93683cfa035c4251bdf2474c3c61b50b,

title = "Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols",

abstract = "Two real-time reverse transcription polymerase chain reaction (rRT-PCR) assays under consideration for deployment to multiple testing laboratories across the USA were evaluated for diagnostic sensitivity and specificity on tissue homogenates obtained from natural and experimental viral hemorrhagic septicemia (VHS)-infected fish. Estimates for diagnostic specificity using virus isolation as the reference method were similar between laboratories regardless of the assay. Diagnostic sensitivity estimates of 0.96 (95% CI: 0.95, 0.97) for Jonstrup et al. (2013)'s assay (J Fish Dis 36:9-23) exceeded the diagnostic sensitivity of 0.85 (95% CI: 0.83, 0.87) for Phelps et al. (2012)'s assay (J Aquat Anim Health 24:238-243). The Jonstrup rRT-PCR assay is robust as demonstrated by high sensitivity and specificity estimates across laboratories and can be used as a valuable tool for targeted surveillance and for testing of suspect VHSV samples.",

keywords = "Diagnostic sensitivity, Diagnostic specificity, Real-time RT-PCR, Surveillance, VHSV",

author = "Warg, {Janet V.} and Travis Clement and Cornwell, {Emily R.} and Angela Cruz and Getchell, {Rodman G.} and Cem Giray and Goodwin, {Andrew E.} and Groocock, {Geoffrey H.} and Mohamed Faisal and Robert Kim and Merry, {Gwenn E.} and Phelps, {Nicholas B.D.} and Reising, {Monica M.} and Isaac Standish and Yan Zhang and Kathy Toohey-Kurth",

year = "2014",

month = aug,

day = "21",

doi = "10.3354/dao02758",

language = "English (US)",

volume = "111",

pages = "15--22",

journal = "Diseases of aquatic organisms",

issn = "0177-5103",

publisher = "Inter-Research",

number = "1",

}

TY - JOUR

T1 - Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols

AU - Warg, Janet V.

AU - Clement, Travis

AU - Cornwell, Emily R.

AU - Cruz, Angela

AU - Getchell, Rodman G.

AU - Giray, Cem

AU - Goodwin, Andrew E.

AU - Groocock, Geoffrey H.

AU - Faisal, Mohamed

AU - Kim, Robert

AU - Merry, Gwenn E.

AU - Phelps, Nicholas B.D.

AU - Reising, Monica M.

AU - Standish, Isaac

AU - Zhang, Yan

AU - Toohey-Kurth, Kathy

PY - 2014/8/21

Y1 - 2014/8/21

N2 - Two real-time reverse transcription polymerase chain reaction (rRT-PCR) assays under consideration for deployment to multiple testing laboratories across the USA were evaluated for diagnostic sensitivity and specificity on tissue homogenates obtained from natural and experimental viral hemorrhagic septicemia (VHS)-infected fish. Estimates for diagnostic specificity using virus isolation as the reference method were similar between laboratories regardless of the assay. Diagnostic sensitivity estimates of 0.96 (95% CI: 0.95, 0.97) for Jonstrup et al. (2013)'s assay (J Fish Dis 36:9-23) exceeded the diagnostic sensitivity of 0.85 (95% CI: 0.83, 0.87) for Phelps et al. (2012)'s assay (J Aquat Anim Health 24:238-243). The Jonstrup rRT-PCR assay is robust as demonstrated by high sensitivity and specificity estimates across laboratories and can be used as a valuable tool for targeted surveillance and for testing of suspect VHSV samples.

AB - Two real-time reverse transcription polymerase chain reaction (rRT-PCR) assays under consideration for deployment to multiple testing laboratories across the USA were evaluated for diagnostic sensitivity and specificity on tissue homogenates obtained from natural and experimental viral hemorrhagic septicemia (VHS)-infected fish. Estimates for diagnostic specificity using virus isolation as the reference method were similar between laboratories regardless of the assay. Diagnostic sensitivity estimates of 0.96 (95% CI: 0.95, 0.97) for Jonstrup et al. (2013)'s assay (J Fish Dis 36:9-23) exceeded the diagnostic sensitivity of 0.85 (95% CI: 0.83, 0.87) for Phelps et al. (2012)'s assay (J Aquat Anim Health 24:238-243). The Jonstrup rRT-PCR assay is robust as demonstrated by high sensitivity and specificity estimates across laboratories and can be used as a valuable tool for targeted surveillance and for testing of suspect VHSV samples.

KW - Diagnostic sensitivity

KW - Diagnostic specificity

KW - Real-time RT-PCR

KW - Surveillance

KW - VHSV

UR - http://www.scopus.com/inward/record.url?scp=84906819050&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84906819050&partnerID=8YFLogxK

U2 - 10.3354/dao02758

DO - 10.3354/dao02758

M3 - Article

C2 - 25144113

AN - SCOPUS:84906819050

SN - 0177-5103

VL - 111

SP - 15

EP - 22

JO - Diseases of aquatic organisms

JF - Diseases of aquatic organisms

IS - 1

ER -

Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols

Abstract

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this