Detection of bacterial β-galactosidase activity in individual Saccharomyces cerevisiae cells by flow cytometry

Friedrich Srienc, Judith L. Campbell, James E. Bailey

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

A fluorogenic substrate and trapping reagent have been used to detect β-galactosidase activity expressed in S. cerevisiae from an Escherichia coli lacZ gene segment encoded on a recombinant plasmid. The measurement may be made in less than 1 msec using a flow cytometer, facilitating rapid experimental characterization of plasmid instability.

Original languageEnglish (US)
Pages (from-to)43-48
Number of pages6
JournalBiotechnology Letters
Volume5
Issue number1
DOIs
StatePublished - Jan 1983

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