TY - JOUR
T1 - Detection of Bartonella henselae DNA in two dogs with pyogranulomatous lymphadenitis
AU - Morales, Sofia C.
AU - Breitschwerdt, Edward B.
AU - Washabau, Robert J.
AU - Matise, Ilze
AU - Maggi, Ricardo G.
AU - Duncan, Ashlee W.
PY - 2007/3/1
Y1 - 2007/3/1
N2 - Case Description - 1 dog evaluated because of inappetence and lameness of the left hind limb of 1 day's duration and 1 dog evaluated because of inappetence, fever, and lymphadenopathy of 2 weeks' duration. Clinical Findings - Histologic examination of excisional biopsy specimens from lymph nodes revealed pyogranulomatous lymphadenitis in both dogs. Quantitative real-time PCR assays detected Bartonella henselae DNA in blood samples and affected lymph node specimens from both dogs. Antibodies against B henselae were not detected via immunofluorescent antibody testing during active disease in either dog. Treatment and Outcome - 1 dog recovered after 6 weeks of treatment with doxycycline (5 mg/kg [2.3 mg/lb], PO, q 12 h), whereas the other dog recovered after receiving a combination of azithromycin (14.5 mg/kg [6.6 mg/lb], PO, q 24 h for 21 days), doxycycline (17.3 mg/kg [7.9 mg/lb], PO, q 24 h for 4 weeks), and immunosuppressive corticosteroid (prednisone [3 mg/kg {1.4 mg/lb}, PO, q 24 h], tapered by decreasing the daily dose by 25% every 2 weeks) treatment. Clinical Relevance - B henselae is implicated as a possible cause or a cofactor in the development of pyogranulomatous lymphadenitis in dogs. In dogs with pyogranulomatous lymphadenitis, immunofluorescent assays may not detect antibodies against B henselae. Molecular testing, including PCR assay of affected tissues, may provide an alternative diagnostic method for detection of B henselae DNA in pyogranulomatous lymph nodes.
AB - Case Description - 1 dog evaluated because of inappetence and lameness of the left hind limb of 1 day's duration and 1 dog evaluated because of inappetence, fever, and lymphadenopathy of 2 weeks' duration. Clinical Findings - Histologic examination of excisional biopsy specimens from lymph nodes revealed pyogranulomatous lymphadenitis in both dogs. Quantitative real-time PCR assays detected Bartonella henselae DNA in blood samples and affected lymph node specimens from both dogs. Antibodies against B henselae were not detected via immunofluorescent antibody testing during active disease in either dog. Treatment and Outcome - 1 dog recovered after 6 weeks of treatment with doxycycline (5 mg/kg [2.3 mg/lb], PO, q 12 h), whereas the other dog recovered after receiving a combination of azithromycin (14.5 mg/kg [6.6 mg/lb], PO, q 24 h for 21 days), doxycycline (17.3 mg/kg [7.9 mg/lb], PO, q 24 h for 4 weeks), and immunosuppressive corticosteroid (prednisone [3 mg/kg {1.4 mg/lb}, PO, q 24 h], tapered by decreasing the daily dose by 25% every 2 weeks) treatment. Clinical Relevance - B henselae is implicated as a possible cause or a cofactor in the development of pyogranulomatous lymphadenitis in dogs. In dogs with pyogranulomatous lymphadenitis, immunofluorescent assays may not detect antibodies against B henselae. Molecular testing, including PCR assay of affected tissues, may provide an alternative diagnostic method for detection of B henselae DNA in pyogranulomatous lymph nodes.
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U2 - 10.2460/javma.230.5.681
DO - 10.2460/javma.230.5.681
M3 - Article
C2 - 17331050
AN - SCOPUS:33847342861
SN - 0003-1488
VL - 230
SP - 681
EP - 685
JO - Journal of the American Veterinary Medical Association
JF - Journal of the American Veterinary Medical Association
IS - 5
ER -