Detection of influenza A virus in swine nasal swab samples with a wash-free magnetic bioassay and a handheld giant magnetoresistance sensing system

Diqing Su; Kai Wu; Venkatramana D. Krishna; Todd Klein; Jinming Liu; Yinglong Feng; Andres M. Perez; Maxim C.J. Cheeran; Jian Ping Wang

doi:10.3389/fmicb.2019.01077

Detection of influenza A virus in swine nasal swab samples with a wash-free magnetic bioassay and a handheld giant magnetoresistance sensing system

Diqing Su, Kai Wu, Venkatramana D. Krishna, Todd Klein, Jinming Liu, Yinglong Feng, Andres M. Perez, Maxim C.J. Cheeran, Jian Ping Wang

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

The dissemination of Influenza A virus (IAV) throughout the world has become one of the main concerns for the health of both animals and human beings. An efficient and sensitive diagnostic tool is thus needed for the early detection of IAV. Here, we developed a wash-free magnetic bioassay and further integrated it with a handheld platform based on giant-magnetoresistance (GMR) sensors. The wash-free magnetic bioassay significantly accelerates and simplifies the detection process. This brand-new system was successful in detecting both IAV nucleoprotein and IAV-contained nasal swab samples from pigs on the farm. The limit of detection (LOD) is 0.3 nM for IAV nucleoprotein and 250 TCID₅₀/mL for IAV-spiked nasal swab samples. The detection of nasal swab samples containing unpurified IAV was also performed, demonstrating the capability of the magnetic wash-free assay in the detection of biomarkers in complex sample matrix.

Original language	English (US)
Article number	1077
Journal	Frontiers in Microbiology
Volume	10
Issue number	MAY
DOIs	https://doi.org/10.3389/fmicb.2019.01077
State	Published - 2019

Bibliographical note

Funding Information:
This work is supported by MNDrive: OVPR STEMMA program, Institute of Engineering in Medicine of the University of Minnesota, College of Veterinary Medicine Emerging, Zoonotic and Infectious Diseases Signature Program, USDA-NIFA, SAES Funds (Project No. MIN-62-097), National Science Foundation MRSEC facility program, the Distinguished McKnight University Professorship, Centennial Chair Professorship, Robert Hartmann Chair Professorship,

Funding Information:
This work is supported by MNDrive: OVPR STEMMA program, Institute of Engineering in Medicine of the University of Minnesota, College of Veterinary Medicine Emerging, Zoonotic and Infectious Diseases Signature Program, USDA-NIFA, SAES Funds (Project No. MIN-62-097), National Science Foundation MRSEC facility program, the Distinguished McKnight University Professorship, Centennial Chair Professorship, Robert Hartmann Chair Professorship, and UROP program from the University of Minnesota. Parts of this work were carried out in the Characterization Facility

Publisher Copyright:
Copyright © 2019 Su, Wu, Krishna, Klein, Liu, Feng, Perez, Cheeran and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Keywords

Giant magnetoresistance
Handheld
Influenza A virus
Magnetic bioassay
Nasal swab sample
On-site diagnosis
Wash-free

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Detection of influenza A virus in swine nasal swab samples with a wash-free magnetic bioassay and a handheld giant magnetoresistance sensing system",

abstract = "The dissemination of Influenza A virus (IAV) throughout the world has become one of the main concerns for the health of both animals and human beings. An efficient and sensitive diagnostic tool is thus needed for the early detection of IAV. Here, we developed a wash-free magnetic bioassay and further integrated it with a handheld platform based on giant-magnetoresistance (GMR) sensors. The wash-free magnetic bioassay significantly accelerates and simplifies the detection process. This brand-new system was successful in detecting both IAV nucleoprotein and IAV-contained nasal swab samples from pigs on the farm. The limit of detection (LOD) is 0.3 nM for IAV nucleoprotein and 250 TCID50/mL for IAV-spiked nasal swab samples. The detection of nasal swab samples containing unpurified IAV was also performed, demonstrating the capability of the magnetic wash-free assay in the detection of biomarkers in complex sample matrix.",

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note = "Funding Information: This work is supported by MNDrive: OVPR STEMMA program, Institute of Engineering in Medicine of the University of Minnesota, College of Veterinary Medicine Emerging, Zoonotic and Infectious Diseases Signature Program, USDA-NIFA, SAES Funds (Project No. MIN-62-097), National Science Foundation MRSEC facility program, the Distinguished McKnight University Professorship, Centennial Chair Professorship, Robert Hartmann Chair Professorship, Funding Information: This work is supported by MNDrive: OVPR STEMMA program, Institute of Engineering in Medicine of the University of Minnesota, College of Veterinary Medicine Emerging, Zoonotic and Infectious Diseases Signature Program, USDA-NIFA, SAES Funds (Project No. MIN-62-097), National Science Foundation MRSEC facility program, the Distinguished McKnight University Professorship, Centennial Chair Professorship, Robert Hartmann Chair Professorship, and UROP program from the University of Minnesota. Parts of this work were carried out in the Characterization Facility Publisher Copyright: Copyright {\textcopyright} 2019 Su, Wu, Krishna, Klein, Liu, Feng, Perez, Cheeran and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.",

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AU - Wu, Kai

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AU - Klein, Todd

AU - Liu, Jinming

AU - Feng, Yinglong

AU - Perez, Andres M.

AU - Cheeran, Maxim C.J.

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N2 - The dissemination of Influenza A virus (IAV) throughout the world has become one of the main concerns for the health of both animals and human beings. An efficient and sensitive diagnostic tool is thus needed for the early detection of IAV. Here, we developed a wash-free magnetic bioassay and further integrated it with a handheld platform based on giant-magnetoresistance (GMR) sensors. The wash-free magnetic bioassay significantly accelerates and simplifies the detection process. This brand-new system was successful in detecting both IAV nucleoprotein and IAV-contained nasal swab samples from pigs on the farm. The limit of detection (LOD) is 0.3 nM for IAV nucleoprotein and 250 TCID50/mL for IAV-spiked nasal swab samples. The detection of nasal swab samples containing unpurified IAV was also performed, demonstrating the capability of the magnetic wash-free assay in the detection of biomarkers in complex sample matrix.

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Detection of influenza A virus in swine nasal swab samples with a wash-free magnetic bioassay and a handheld giant magnetoresistance sensing system

Abstract

Bibliographical note

Keywords

UN SDGs

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