The frequency of Mycobacterium bovis detection in milk samples obtained from infected animals was explored in an intensive dairy area in Argentina. To this end, an "in house" polymerase chain reaction (PCR) method was developed using Mycobacterium tuberculosis Complex specific INS1-INS2 primers, and its performance was compared with that of bacteriological methods. The decontamination procedures previous to culture reduced M. bovis viability. The pathogen was identified in milk samples from 1 of 143 infected cows and in none of 43 uninfected ones. Even though PCR sensitivity was found to be 2-20 times higher than that of bacteriology in experimentally inoculated milk samples, all 186 field samples resulted negative by PCR, including the bacteriologically-confirmed one. In spite of the high prevalence of bovine tuberculosis in Argentinean dairy herds, the detection of M. bovis in milk is an unusual finding.
|Translated title of the contribution||Detection of Mycobacterium bovis in milk by bacteriological methods and polymerase chain reaction|
|Number of pages||7|
|Journal||Revista Argentina de Microbiologia|
|State||Published - Apr 17 2002|
- Bovine tuberculosis
- Mycobacterium bovis
- Polymerase chain reaction