Random-amplified polymorphic DNA (RAPD) markers were used to determine genetic relationships among Phaseolus vulgaris breeding populations. Genetic distances were calculated from the distribution of 317 RAPD markers among 8 parents, 10 individuals from 8 cycle-one populations, 10 individuals from 6 cycle-two populations and 10 individuals from 2 cycle-three populations of a conical cross. Genetic distances between populations and parents were consistent with their degree of relationship in the crossing scheme indicating that a RAPD analysis is a sensitive and useful method for categorizing breeding materials according to their genetic similarities. Genetic variation among individuals within populations increased from cycle one to cycle three and variation among populations within the cycles decreased from cycle one to cycle three in the conical cross. The results showed that this crossing scheme can be used to collect the genetic diversity in eight parents into a single plant breeding population.
Bibliographical noteFunding Information:
This research was supported by a NSERC/Agriculture-Canada grant to T.E.M., K.P.P; and L.R. Erickson. We thank J. Wilkins for help with primer screening and gratefully acknowledge the assistance of Angela Hill and Jean Wolting in preparing the manuscript.
Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.
- Common bean
- Conical cross
- Genetic distance
- RAPD markers