Determination of hemoglobin and serum albumin adducts of benzo[a]pyrene by gas chromatography-mass spectrometry in humans and their relation to exposure and to other biological markers

A. A. Melikian, S. Malpure, A. John, M. Meng, B. Schoket, G. Mayer, I. Vincze, A. Kolozsi-Ringelhann, S. S. Hecht

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

In this study we have examined the relationship of benzo[a]pyrene (B[a]P) exposure with B[a]P-7,8-diol-9,10-epoxide (BPDE) adduct formation in globin and serum albumin (SA). We also have evaluated the relationship of BPDE-globin and -SA adduct formation with urinary 1-hydroxypyrene (1-OH-pyrene), and bulky DNA adducts in peripheral blood lymphocytes. The study groups consist of male Hungarian garage mechanics (smokers and nonsmokers) exposed to diesel engine exhaust and matched control samples from a blood bank in Budapest. The mean level of BPDE-globin adducts in the garage mechanics (n=15) is 5.9±4.8 fmol/mg globin (ranging from 0.5 to 19.9 fmol/mg globin) and that in controls (n=15), is 2.4±2.5 fmol/mg globin (ranging from ND to 8.5 fmol BPDE/mg globin). The mean of BPDE-SA in garage mechanics is 0.36±0.6 fmol/mg SA (ranging from ND to 2.6 fmol/mg SA) and, in controls it is 0.29±0.3 fmol/mg SA (ranging from ND to 1.3 fmol/mg SA). The results of this study indicate that levels of BPDE-SA adducts are about one order of magnitude lower than levels of globin adducts. The mean of BPDE-globin and -SA adducts in garage mechanics are 2.4-fold (p=0.02) and 1.2-fold (p=0.7) higher than those in controls, respectively. Similarly, levels of urinary 1-OH-pyrene in the exposed group are 2.1-fold higher than those in controls (p=0.03). The correlation coefficients between estimated B[a]P exposure (both ingested and inhaled) and formation of globin adducts and urinary 1-OH-pyrene are r=0.49 (p=0.06), and r=0.31 (p=0.26), respectively. This study suggests that BPDE-globin adducts and urinary 1-OH-pyrene may be better biomarkers of exposure than BPDE-SA adducts.

Original languageEnglish (US)
Pages (from-to)125-134
Number of pages10
JournalPolycyclic Aromatic Compounds
Volume17
Issue number1-4
DOIs
StatePublished - 1999

Bibliographical note

Funding Information:
States National Cancer Institute and a grant from the Hungarian Ministry of Welfare. The assistance of Dr. B. Latki, Mrs. V. Szalai, A. Karacsonyi, G. Papp and K. Levai in the sample collections is gratefully acknowledged.

Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

Keywords

  • B[a]P-globin adducts
  • B[a]P-serum albumin adducts
  • GC-MS
  • Urinary 1-OH-pyrene

Fingerprint Dive into the research topics of 'Determination of hemoglobin and serum albumin adducts of benzo[a]pyrene by gas chromatography-mass spectrometry in humans and their relation to exposure and to other biological markers'. Together they form a unique fingerprint.

Cite this