TY - JOUR
T1 - Determination of quinolone antibiotics in growth media by reversed- phase high-performance liquid chromatography
AU - Wright, David H.
AU - Herman, Varen K.
AU - Konstantinides, Frank N.
AU - Rotschafer, John C.
PY - 1998/5/8
Y1 - 1998/5/8
N2 - A simple, accurate, precise, and versatile high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of three quinolone antibiotics in Mueller-Hinton broth. The fluoroquinolone agents studied were ciprofloxacin, ofloxacin, and sparfloxacin; other quinolone agents have been identified using this method but not validated in this matrix (levofloxacin, clinafloxacin, temafloxacin, and trovafloxacin). In addition, several other biological growth mediums have been investigated (human serum, human urine, Todd-Hewitt growth media, Ensure enteral feeding solution, and Haemophilus growth media). This method uses UV detection (280 nm), a simple, one-step protein precipitation extraction, and separation using a C18 column with an isocratic, ion-pairing mobile phase. An appropriate internal standard was obtained by using another quinolone antibiotic of differing retention time. The calibration curves were linear (r2 ≤ 0.999) over a concentration range of 0.0625-20.0 μg/ml with a lower limit of quantification of 0.1 μg/ml. The intra-day and inter-day coefficients of variation were less than 15%.
AB - A simple, accurate, precise, and versatile high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of three quinolone antibiotics in Mueller-Hinton broth. The fluoroquinolone agents studied were ciprofloxacin, ofloxacin, and sparfloxacin; other quinolone agents have been identified using this method but not validated in this matrix (levofloxacin, clinafloxacin, temafloxacin, and trovafloxacin). In addition, several other biological growth mediums have been investigated (human serum, human urine, Todd-Hewitt growth media, Ensure enteral feeding solution, and Haemophilus growth media). This method uses UV detection (280 nm), a simple, one-step protein precipitation extraction, and separation using a C18 column with an isocratic, ion-pairing mobile phase. An appropriate internal standard was obtained by using another quinolone antibiotic of differing retention time. The calibration curves were linear (r2 ≤ 0.999) over a concentration range of 0.0625-20.0 μg/ml with a lower limit of quantification of 0.1 μg/ml. The intra-day and inter-day coefficients of variation were less than 15%.
KW - Ciprofloxacin
KW - Ofloxacin
KW - Sparfloxacin
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UR - http://www.scopus.com/inward/citedby.url?scp=0032496212&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(98)00006-1
DO - 10.1016/S0378-4347(98)00006-1
M3 - Article
C2 - 9653930
AN - SCOPUS:0032496212
SN - 1572-6495
VL - 709
SP - 97
EP - 104
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1
ER -