The current study was aimed to generate mouse monoclonal antibodies (mAbs) specific for Fc receptor common γ chain (FcRγ), which serves a critical signaling molecule for numerous human immune receptors that play essential roles in human immune responses. Two BALB/c mice were immunized with the synthesized polypeptides containing 19 residues at the carboxyl terminus of human FcR γ chain. The spleen cells from the immunized mice were fused with myeloma Sp2/0 cells. ELISA was used to screen mAbs and the positive clones were selected for further characterization with Western blot and flow cytometry assays. After screen, there hybridoma cell lines which secreted mAbs specifically recognized the FcR γ chain were obtained and named as 5B6, 7D3 and 8D4. The 7D3 mAb had the best quality and it could recognize the FcR γ chain in vivo and in vitro with 2.5 μg/mL. We have generated the FcRγ-specific mAbs, which may serve as a valuable tool in the functional studies of FcRγ associated immune receptors.
|Original language||English (US)|
|Number of pages||4|
|Journal||Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology|
|State||Published - Jul 2011|