Development and validation of a LC–MS/MS method for the quantification of tenofovir and emtricitabine in seminal plasma

S. M. Illamola, E. Valade, D. Hirt, E. Dulioust, Y. Zheng, J. P. Wolf, J. M. Tréluyer

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Accurate and sensitive liquid-chromatography tandem mass spectrometry method for the quantification of tenofovir and emtricitabine in seminal plasma has been developed and full validated. Molecules were separated by high-performance liquid chromatography on an Atlantis T3 C18 column using a gradient of deionized water and methanol, including 0.05% formic acid (250 μl/min) and detected by electrospray ionisation/tandem mass spectrometry in positive ion mode. The method was validated over a clinical range of 3.13–1000 ng/mL for tenofovir and 6.25–2000 ng/mL for emtricitabine. Inter and intra-assay precisions were <9.37% for tenofovir and<10.88% for emtricitabine, and accuracies were between 0.48% and 8.43% for tenofovir, and between 0.64% and 13.87% for emtricitabine. The developed method was successfully applied for analysing tenofovir and emtricitabine concentrations in seminal plasma samples from a clinical study. The use of tandem mass spectrometry can be a suitable method for the analysis of this kind of matrices, providing high sensitivity and specificity to the analysis.

Original languageEnglish (US)
Pages (from-to)234-241
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume1033-1034
DOIs
StatePublished - Oct 15 2016
Externally publishedYes

Keywords

  • Emtricitabine
  • LC–MS/MS
  • Seminal plasma
  • Tenofovir

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