Abstract
Fusarium head blight (FHB) is a devastating wheat disease with a significant economic impact. Fhb1 is the most important large effect and stable QTL for FHB resistance. A pore-forming toxin-like (PFT) gene was recently identified as an underlying gene for Fhb1 resistance. In this study, we developed and validated a PFT-based Kompetitive allele specific PCR (KASP) marker for Fhb1. The KASP marker, PFT_KASP, was used to screen 298 diverse wheat breeding lines and cultivars. The KASP clustering results were compared with gel-based gene specific markers and the widely used linked STS marker, UMN10. Eight disagreements were found between PFT_KASP and UMN10 assays among the tested lines. Based on the genotyping and sequencing of genes in the Fhb1 region, these genotypes were found to be common with a previously characterized susceptible haplotype. Therefore, our results indicate that PFT_ KASP is a perfect diagnostic marker for Fhb1 and would be a valuable tool for introgression and pyramiding of FHB resistance in wheat cultivars.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 200-207 |
| Number of pages | 8 |
| Journal | Plant Pathology Journal |
| Volume | 35 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2019 |
Bibliographical note
Funding Information:This work was supported by the USDA National Institute of Food and Agriculture, Hatch project 1016879 and Maryland Agricultural Experiment Station via MAES Grant No. 2956952.
Publisher Copyright:
© The Korean Society of Plant Pathology.
Keywords
- Fhb1
- Fusarium head blight
- KASP assay
- Pore-forming toxin-like (PFT)
- Wheat