Development of a multiplexed giant magnetoresistive biosensor array prototype to quantify ovarian cancer biomarkers

Todd Klein; Wei Wang; Lina Yu; Kai Wu; Kristin L.M. Boylan; Rachel Isaksson Vogel; Amy P.N. Skubitz; Jian Ping Wang

doi:10.1016/j.bios.2018.10.046

Development of a multiplexed giant magnetoresistive biosensor array prototype to quantify ovarian cancer biomarkers

Todd Klein, Wei Wang, Lina Yu, Kai Wu, Kristin L.M. Boylan, Rachel Isaksson Vogel, Amy P.N. Skubitz, Jian Ping Wang

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

In this work, we developed benchtop and handheld Giant Magnetoresistive (GMR) biosensing systems that serve as platforms for detecting a wide variety of protein biomarkers for human diseases. System development included spintronic and nanomagnetic materials, biomolecular chemistry, electronic circuitry, analog and digital signal processing, firmware programming, user interface programming on both PC and Android smartphone, communications over both USB and Bluetooth, and mechanical integration. In this work, we demonstrated the benchtop GMR biosensing system in the context of ovarian cancer assay development. The prototype system delivered the required performance in terms of high-sensitivity multiplex assays in a portable format with enough flexibility to serve as a platform for ovarian cancer and many other diseases. We achieved multiplex detection of cancer antigen 125 (CA125 II), human epididymis protein 4 (HE4), and interleukin 6 (IL6), with limits of detection (LOD) as low as 3.7 U/mL, 7.4 pg/mL, and 7.4 pg/mL, respectively.

Original language	English (US)
Pages (from-to)	301-307
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	126
DOIs	https://doi.org/10.1016/j.bios.2018.10.046
State	Published - Feb 1 2019

Bibliographical note

Funding Information:
We acknowledge XPRIZE Foundation and Nokia Sensing XCHALLENGE competition for motivating the design of the z-Lab Diagnosis Platform which won a Distinguished Prize Award. This work was supported in part by an Ovarian Cancer Translational Pilot Award (W81XWH-11-1-0496) through the U.S. Department of Defense. Additional funding came both from the Jan Chorzempa Cancer Research Endowed Fund (11726) though the University of Minnesota Foundation and from a Doctoral Dissertation Fellowship through the University of Minnesota Graduate School. Parts of this work were carried out in the Characterization Facility, University of Minnesota, a member of the NSF-funded Materials Research Facilities Network (www.mrfn.org) via the MRSEC program.

Funding Information:
We acknowledge XPRIZE Foundation and Nokia Sensing XCHALLENGE competition for motivating the design of the z-Lab Diagnosis Platform which won a Distinguished Prize Award. This work was supported in part by an Ovarian Cancer Translational Pilot Award (W81XWH-11-1-0496) through the U.S. Department of Defense . Additional funding came both from the Jan Chorzempa Cancer Research Endowed Fund (11726) though the University of Minnesota Foundation and from a Doctoral Dissertation Fellowship through the University of Minnesota Graduate School. Parts of this work were carried out in the Characterization Facility, University of Minnesota , a member of the NSF-funded Materials Research Facilities Network ( www.mrfn.org ) via the MRSEC program.

Publisher Copyright:
© 2018 Elsevier B.V.

Keywords

Biomarkers
CA125
Giant magnetoresistance
HE4
Hand-held system
IL6
Multiplex detection
Ovarian cancer

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abstract = "In this work, we developed benchtop and handheld Giant Magnetoresistive (GMR) biosensing systems that serve as platforms for detecting a wide variety of protein biomarkers for human diseases. System development included spintronic and nanomagnetic materials, biomolecular chemistry, electronic circuitry, analog and digital signal processing, firmware programming, user interface programming on both PC and Android smartphone, communications over both USB and Bluetooth, and mechanical integration. In this work, we demonstrated the benchtop GMR biosensing system in the context of ovarian cancer assay development. The prototype system delivered the required performance in terms of high-sensitivity multiplex assays in a portable format with enough flexibility to serve as a platform for ovarian cancer and many other diseases. We achieved multiplex detection of cancer antigen 125 (CA125 II), human epididymis protein 4 (HE4), and interleukin 6 (IL6), with limits of detection (LOD) as low as 3.7 U/mL, 7.4 pg/mL, and 7.4 pg/mL, respectively.",

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Development of a multiplexed giant magnetoresistive biosensor array prototype to quantify ovarian cancer biomarkers

Abstract

Bibliographical note

Keywords

UN SDGs

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