Development of microsatellite loci for the western slimy salamander (Plethodon albagula) using 454 sequencing

Brett N. Spatola; William E. Peterman; Nathan T. Stephens; Grant M. Connette; Donald B. Shepard; Kenneth H. Kozak; Raymond D. Semlitsch; Lori S. Eggert

doi:10.1007/s12686-012-9784-5

Development of microsatellite loci for the western slimy salamander (Plethodon albagula) using 454 sequencing

Brett N. Spatola, William E. Peterman, Nathan T. Stephens, Grant M. Connette, Donald B. Shepard, Kenneth H. Kozak, Raymond D. Semlitsch, Lori S. Eggert

Fisheries, Wildlife, and Conservation Biology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Terrestrial salamanders of the genus Plethodon are closely tied to abiotic environmental habitat features and are particularly sensitive to land use and habitat change. To better understand the effects of land use and habitat fragmentation on population genetic characteristics, we screened 137 primers developed from 454 sequence libraries, and developed 27 microsatellite primers for the western slimy salamander, Plethodon albagula. Twenty-two of these primers cross-amplified in P. shermani. These new loci will be invaluable tools to study habitat variables affecting gene flow, population genetic structure, and genetic diversity across heterogeneous landscapes.

Original language	English (US)
Pages (from-to)	267-270
Number of pages	4
Journal	Conservation Genetics Resources
Volume	5
Issue number	1
DOIs	https://doi.org/10.1007/s12686-012-9784-5
State	Published - Mar 2013

Bibliographical note

Funding Information:
Acknowledgments We thank C. Cramer (ASU) for sharing 454 sequences for P. albagula, and funding from the University of Missouri Research Board (CB000402), the DoD Strategic Environmental Research and Development Program (RC2155), the Arkansas Biosciences Institute (research component of Arkansas Tobacco Settlement Proceeds Act of 2000), National Science Foundation (DEB-0949590 to KHK), and S.E. Trauth for Arkansas field site access.

Keywords

Landscape genetics
Plethodon shermani
Population genetics

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title = "Development of microsatellite loci for the western slimy salamander (Plethodon albagula) using 454 sequencing",

abstract = "Terrestrial salamanders of the genus Plethodon are closely tied to abiotic environmental habitat features and are particularly sensitive to land use and habitat change. To better understand the effects of land use and habitat fragmentation on population genetic characteristics, we screened 137 primers developed from 454 sequence libraries, and developed 27 microsatellite primers for the western slimy salamander, Plethodon albagula. Twenty-two of these primers cross-amplified in P. shermani. These new loci will be invaluable tools to study habitat variables affecting gene flow, population genetic structure, and genetic diversity across heterogeneous landscapes.",

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note = "Funding Information: Acknowledgments We thank C. Cramer (ASU) for sharing 454 sequences for P. albagula, and funding from the University of Missouri Research Board (CB000402), the DoD Strategic Environmental Research and Development Program (RC2155), the Arkansas Biosciences Institute (research component of Arkansas Tobacco Settlement Proceeds Act of 2000), National Science Foundation (DEB-0949590 to KHK), and S.E. Trauth for Arkansas field site access.",

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doi = "10.1007/s12686-012-9784-5",

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AU - Spatola, Brett N.

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AU - Stephens, Nathan T.

AU - Connette, Grant M.

AU - Shepard, Donald B.

AU - Kozak, Kenneth H.

AU - Semlitsch, Raymond D.

AU - Eggert, Lori S.

N1 - Funding Information: Acknowledgments We thank C. Cramer (ASU) for sharing 454 sequences for P. albagula, and funding from the University of Missouri Research Board (CB000402), the DoD Strategic Environmental Research and Development Program (RC2155), the Arkansas Biosciences Institute (research component of Arkansas Tobacco Settlement Proceeds Act of 2000), National Science Foundation (DEB-0949590 to KHK), and S.E. Trauth for Arkansas field site access.

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N2 - Terrestrial salamanders of the genus Plethodon are closely tied to abiotic environmental habitat features and are particularly sensitive to land use and habitat change. To better understand the effects of land use and habitat fragmentation on population genetic characteristics, we screened 137 primers developed from 454 sequence libraries, and developed 27 microsatellite primers for the western slimy salamander, Plethodon albagula. Twenty-two of these primers cross-amplified in P. shermani. These new loci will be invaluable tools to study habitat variables affecting gene flow, population genetic structure, and genetic diversity across heterogeneous landscapes.

AB - Terrestrial salamanders of the genus Plethodon are closely tied to abiotic environmental habitat features and are particularly sensitive to land use and habitat change. To better understand the effects of land use and habitat fragmentation on population genetic characteristics, we screened 137 primers developed from 454 sequence libraries, and developed 27 microsatellite primers for the western slimy salamander, Plethodon albagula. Twenty-two of these primers cross-amplified in P. shermani. These new loci will be invaluable tools to study habitat variables affecting gene flow, population genetic structure, and genetic diversity across heterogeneous landscapes.

KW - Landscape genetics

KW - Plethodon shermani

KW - Population genetics

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Development of microsatellite loci for the western slimy salamander (Plethodon albagula) using 454 sequencing

Abstract

Bibliographical note

Keywords

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