Development of mPing-based activation tags for crop insertional mutagenesis

Alexander Johnson; Edward Mcassey; Stephanie Diaz; Jacob Reagin; Priscilla S. Redd; Daymond R. Parrilla; Hanh Nguyen; Adrian Stec; Lauren A.L. McDaniel; Thomas E. Clemente; Robert M. Stupar; Wayne A. Parrott; C. Nathan Hancock

doi:10.1002/pld3.300

Development of mPing-based activation tags for crop insertional mutagenesis

Alexander Johnson, Edward Mcassey, Stephanie Diaz, Jacob Reagin, Priscilla S. Redd, Daymond R. Parrilla, Hanh Nguyen, Adrian Stec, Lauren A.L. McDaniel, Thomas E. Clemente, Robert M. Stupar, Wayne A. Parrott, C. Nathan Hancock

Agronomy and Plant Genetics

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to induce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing-based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. Expression analysis of a line that contained a heritable insertion of the mmPing20F activation tag indicated that the activation tag induced overexpression of the nearby soybean genes. This represents a significant advance in gene discovery technology as activation tags have the potential to induce more phenotypes than the original mPing element, improving the overall effectiveness of the mutagenesis system.

Original language	English (US)
Article number	e00300
Journal	Plant Direct
Volume	5
Issue number	1
DOIs	https://doi.org/10.1002/pld3.300
State	Published - Jan 2021

Bibliographical note

Publisher Copyright:
© 2021 The Authors. Plant Direct published by American Society of Plant Biologists, Society for Experimental Biology and John Wiley & Sons Ltd.

Keywords

activation tagging
gene discovery
soybean
transposable element

Access

10.1002/pld3.300

OpenUrl availability

Full text

Cite this

@article{752977d939da4c1e9c926da3a97adf2d,

title = "Development of mPing-based activation tags for crop insertional mutagenesis",

abstract = "Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to induce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing-based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. Expression analysis of a line that contained a heritable insertion of the mmPing20F activation tag indicated that the activation tag induced overexpression of the nearby soybean genes. This represents a significant advance in gene discovery technology as activation tags have the potential to induce more phenotypes than the original mPing element, improving the overall effectiveness of the mutagenesis system.",

keywords = "activation tagging, gene discovery, soybean, transposable element",

author = "Alexander Johnson and Edward Mcassey and Stephanie Diaz and Jacob Reagin and Redd, {Priscilla S.} and Parrilla, {Daymond R.} and Hanh Nguyen and Adrian Stec and McDaniel, {Lauren A.L.} and Clemente, {Thomas E.} and Stupar, {Robert M.} and Parrott, {Wayne A.} and Hancock, {C. Nathan}",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors. Plant Direct published by American Society of Plant Biologists, Society for Experimental Biology and John Wiley & Sons Ltd.",

year = "2021",

month = jan,

doi = "10.1002/pld3.300",

language = "English (US)",

volume = "5",

journal = "Plant Direct",

issn = "2475-4455",

publisher = "John Wiley and Sons Inc.",

number = "1",

}

TY - JOUR

T1 - Development of mPing-based activation tags for crop insertional mutagenesis

AU - Johnson, Alexander

AU - Mcassey, Edward

AU - Diaz, Stephanie

AU - Reagin, Jacob

AU - Redd, Priscilla S.

AU - Parrilla, Daymond R.

AU - Nguyen, Hanh

AU - Stec, Adrian

AU - McDaniel, Lauren A.L.

AU - Clemente, Thomas E.

AU - Stupar, Robert M.

AU - Parrott, Wayne A.

AU - Hancock, C. Nathan

PY - 2021/1

Y1 - 2021/1

N2 - Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to induce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing-based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. Expression analysis of a line that contained a heritable insertion of the mmPing20F activation tag indicated that the activation tag induced overexpression of the nearby soybean genes. This represents a significant advance in gene discovery technology as activation tags have the potential to induce more phenotypes than the original mPing element, improving the overall effectiveness of the mutagenesis system.

AB - Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to induce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing-based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. Expression analysis of a line that contained a heritable insertion of the mmPing20F activation tag indicated that the activation tag induced overexpression of the nearby soybean genes. This represents a significant advance in gene discovery technology as activation tags have the potential to induce more phenotypes than the original mPing element, improving the overall effectiveness of the mutagenesis system.

KW - activation tagging

KW - gene discovery

KW - soybean

KW - transposable element

UR - http://www.scopus.com/inward/record.url?scp=85100257678&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85100257678&partnerID=8YFLogxK

U2 - 10.1002/pld3.300

DO - 10.1002/pld3.300

M3 - Article

C2 - 33506165

AN - SCOPUS:85100257678

SN - 2475-4455

VL - 5

JO - Plant Direct

JF - Plant Direct

IS - 1

M1 - e00300

ER -

Development of mPing-based activation tags for crop insertional mutagenesis

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this