Development of peptide-selected CD8 T cells in fetal thymic organ culture occurs via the conventional pathway

Kristin A Hogquist, Jody L. Bonnevier

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Fetal thymic organ culture of TCR transgenic (Tg) tissue has been used to study issues of timing and specificity in T cell development. Because most TCR Tgs express a rearranged αβ TCR on the cell surface at an earlier stage in development than normal mice, there is a possibility that the conclusions of studies using TCR Tg cultures may not apply to normal development. In particular, in our studies of peptide-induced development of CD8 T cells, it is possible that the peptide acts on the immature double-negative cell, driving development of CD8 T cells without passing through a double-positive stage. This issue was examined by asking whether MHC class I restriction was required and by analyzing CD8β levels and endogenous TCRα chain rearrangements. We found that if nonstimulatory peptides were used in fetal thymic organ culture, CD8 T cells developed via the conventional pathway, transiting through a double-positive stage. However, we could not rule out that cells selected in the presence of stimulatory peptides (agonists) did not develop directly from double-negative precursors.

Original languageEnglish (US)
Pages (from-to)3896-3901
Number of pages6
JournalJournal of Immunology
Volume161
Issue number8
StatePublished - Oct 15 1998

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