TY - JOUR
T1 - Development of polymerase chain reaction-based assays for bacterial gene detection
AU - Johnson, James R.
PY - 2000/8
Y1 - 2000/8
N2 - Polymerase chain reaction-based assays provide rapid, simple, and sensitive detection of bacterial genes, but are not without their drawbacks. This review summarizes the principal advantages and disadvantages of PCR- based bacterial gene detection, provides guidelines for the development and validation of new PCR assays, and describes potential pitfalls that may be encountered and how these can be avoided. (C) 2000 Published by Elsevier Science B.V.
AB - Polymerase chain reaction-based assays provide rapid, simple, and sensitive detection of bacterial genes, but are not without their drawbacks. This review summarizes the principal advantages and disadvantages of PCR- based bacterial gene detection, provides guidelines for the development and validation of new PCR assays, and describes potential pitfalls that may be encountered and how these can be avoided. (C) 2000 Published by Elsevier Science B.V.
KW - Polymerase chain reaction (PCR)
KW - Primer design
KW - Review
UR - http://www.scopus.com/inward/record.url?scp=0033848704&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033848704&partnerID=8YFLogxK
U2 - 10.1016/S0167-7012(00)00160-3
DO - 10.1016/S0167-7012(00)00160-3
M3 - Review article
C2 - 10958965
AN - SCOPUS:0033848704
SN - 0167-7012
VL - 41
SP - 201
EP - 209
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
IS - 3
ER -