Dexamethasone inhibits mucous glycoprotein secretion via a phospholipase A2-dependent mechanism in cultured chinchilla middle ear epithelial cells

Jizhen Lin; Steven K. Juhn; George L. Adams; G. Scott Giebink; Youngki Kim

doi:10.3109/00016489709113413

Dexamethasone inhibits mucous glycoprotein secretion via a phospholipase A₂-dependent mechanism in cultured chinchilla middle ear epithelial cells

Jizhen Lin, Steven K. Juhn, George L. Adams, G. Scott Giebink, Youngki Kim

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Inhibition or attenuation of mucous hypersecretion in middle ear epithelium is a key step toward resolution of mucoid otitis media. Mucous hypersecretion induced by platelet-activating factor (PAF) in cultured Chinchilla middle ear epithelial cells is dependent on arachidonic acid metabolites via PAF receptors, suggestive of the role of phospholipase A₂ (PLA₂) in mucous glycoprotein (MGP) secretion. In this study, dexamethasone added to cultured Chinchilla middle ear epithelial cells inhibited baseline and PAF-induced MGP secretion in a concentration-dependent manner. A definite time lag (16 h) was observed between administration of dexamethasone and MGP inhibition. This inhibition was reversed by the addition of exogenous PEA₂ (the rate-limiting enzyme of arachidonic acid metabolism) and actinomycin D (an inhibitor of mRNA synthesis). This suggests that dexamethasone inhibits baseline and PAF-induced MGP secretion via a PLA₂-dependent mechanism.

Original language	English (US)
Pages (from-to)	406-413
Number of pages	8
Journal	Acta Oto-Laryngologica
Volume	117
Issue number	3
DOIs	https://doi.org/10.3109/00016489709113413
State	Published - 1997

Bibliographical note

Funding Information:
This study was supported by research grant number 5 PO1 DC 00133 from the National Institute on Deafness and Other Communication Disorders, National Institutes of Health.

Keywords

PLA middle ear epithelial cells in vitro
dexamethasone
mucous glycoprotein
platelet-activating factor

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title = "Dexamethasone inhibits mucous glycoprotein secretion via a phospholipase A2-dependent mechanism in cultured chinchilla middle ear epithelial cells",

abstract = "Inhibition or attenuation of mucous hypersecretion in middle ear epithelium is a key step toward resolution of mucoid otitis media. Mucous hypersecretion induced by platelet-activating factor (PAF) in cultured Chinchilla middle ear epithelial cells is dependent on arachidonic acid metabolites via PAF receptors, suggestive of the role of phospholipase A2 (PLA2) in mucous glycoprotein (MGP) secretion. In this study, dexamethasone added to cultured Chinchilla middle ear epithelial cells inhibited baseline and PAF-induced MGP secretion in a concentration-dependent manner. A definite time lag (16 h) was observed between administration of dexamethasone and MGP inhibition. This inhibition was reversed by the addition of exogenous PEA2 (the rate-limiting enzyme of arachidonic acid metabolism) and actinomycin D (an inhibitor of mRNA synthesis). This suggests that dexamethasone inhibits baseline and PAF-induced MGP secretion via a PLA2-dependent mechanism.",

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author = "Jizhen Lin and Juhn, {Steven K.} and Adams, {George L.} and {Scott Giebink}, G. and Youngki Kim",

note = "Funding Information: This study was supported by research grant number 5 PO1 DC 00133 from the National Institute on Deafness and Other Communication Disorders, National Institutes of Health.",

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AU - Lin, Jizhen

AU - Juhn, Steven K.

AU - Adams, George L.

AU - Scott Giebink, G.

AU - Kim, Youngki

N1 - Funding Information: This study was supported by research grant number 5 PO1 DC 00133 from the National Institute on Deafness and Other Communication Disorders, National Institutes of Health.

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N2 - Inhibition or attenuation of mucous hypersecretion in middle ear epithelium is a key step toward resolution of mucoid otitis media. Mucous hypersecretion induced by platelet-activating factor (PAF) in cultured Chinchilla middle ear epithelial cells is dependent on arachidonic acid metabolites via PAF receptors, suggestive of the role of phospholipase A2 (PLA2) in mucous glycoprotein (MGP) secretion. In this study, dexamethasone added to cultured Chinchilla middle ear epithelial cells inhibited baseline and PAF-induced MGP secretion in a concentration-dependent manner. A definite time lag (16 h) was observed between administration of dexamethasone and MGP inhibition. This inhibition was reversed by the addition of exogenous PEA2 (the rate-limiting enzyme of arachidonic acid metabolism) and actinomycin D (an inhibitor of mRNA synthesis). This suggests that dexamethasone inhibits baseline and PAF-induced MGP secretion via a PLA2-dependent mechanism.

AB - Inhibition or attenuation of mucous hypersecretion in middle ear epithelium is a key step toward resolution of mucoid otitis media. Mucous hypersecretion induced by platelet-activating factor (PAF) in cultured Chinchilla middle ear epithelial cells is dependent on arachidonic acid metabolites via PAF receptors, suggestive of the role of phospholipase A2 (PLA2) in mucous glycoprotein (MGP) secretion. In this study, dexamethasone added to cultured Chinchilla middle ear epithelial cells inhibited baseline and PAF-induced MGP secretion in a concentration-dependent manner. A definite time lag (16 h) was observed between administration of dexamethasone and MGP inhibition. This inhibition was reversed by the addition of exogenous PEA2 (the rate-limiting enzyme of arachidonic acid metabolism) and actinomycin D (an inhibitor of mRNA synthesis). This suggests that dexamethasone inhibits baseline and PAF-induced MGP secretion via a PLA2-dependent mechanism.

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