Different Toxic Effects of Hydrogen Peroxide, Nitric Oxide, and Superoxide on Human, Pig, and Rat Islets of Langerhans

T. Wacker, H. Jahr, S. Weinand, H. Brandhorst, D. Brandhorst, D. Lau, B. J. Hering, K. Federlin, R. G. Bretzel

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Susceptibility of islet cells to damage by hydrogen peroxide, superoxide, and nitric oxide was determined on islets isolated from humans, pigs, and rats. Islets were incubated for 20 hr at 37°C with different concentrations of hydrogen peroxide, hypoxanthine/xanthine oxidase, or nitroprusside sodium, respectively. Islet cell damage was then measured as trypan blue-uptake. Rat islets showed a higher sensitivity than human or pig islets to damage by reactive oxygen species or nitric oxide. These results indicate that pig islets may be a more suitable model than rat islets to study inflammatory islet cell damage in diabetes and clinical islet transplantation.

Original languageEnglish (US)
Pages (from-to)133-135
Number of pages3
JournalExperimental and Clinical Endocrinology and Diabetes
Volume103
DOIs
StatePublished - Jan 1 1995

Keywords

  • hydrogen peroxide
  • islet of Langerhans
  • nitric oxide
  • superoxide

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