TY - JOUR
T1 - Differential activation of murine macrophages by angelan and LPS
AU - Jeon, Young Jin
AU - Han, Sang Bae
AU - Ahn, Kyung Seop
AU - Kim, Hwan Mook
PY - 2000
Y1 - 2000
N2 - In our previous studies, we showed that angelan, a polysaccharide purified from Angelica gigas Nakai, is a potent LPS-mimetic in murine macrophages [Jeon, Y.J., Han, S.B., Ahn, K.S., Kim, H.M., 1999. Activation of NF-κB/Rel in angelan-stimulated macrophages. Immunopharmacology 43, 1-9]. Angelan stimulates murine macrophage to produce cytokines including iNOS and activate NF-κB/Rel. In the present study, we investigated the role of CD14 and complement receptor type 3 (CR3) in mediating NO production and NF- κB/Rel activation induced by angelan and LPS. Three major differences between angelan and LPS were observed. First, angelan does not require serum proteins for NO response and NF-κB/Rel activation, while the activation by LPS requires serum proteins. Second, blocking of either CD14 or CR3 decreased angelan-induced NO response, while LPS-mediated NO production was inhibited by anti-CD14 mAb only. Third, angelan induced strong NF-κB/Rel and slight AP-1 DNA binding, whereas LPS potently activated both NF-κB/Rel and AP-1. Both angelan and LPS degraded IκB proteins and subsequently induced the mobilization of NF-κB/Rel proteins (p65, c-rel and p50) into nucleus. This suggests that macrophages display a common signaling machinery leading to the NF-κB/Rel activation in response to different stimulants. In conclusion, angelan and LPS use the membrane receptor CD14 and CR3 differentially for signaling NF-κB/Rel activation and NO production. (C) 2000 Elsevier Science B.V.
AB - In our previous studies, we showed that angelan, a polysaccharide purified from Angelica gigas Nakai, is a potent LPS-mimetic in murine macrophages [Jeon, Y.J., Han, S.B., Ahn, K.S., Kim, H.M., 1999. Activation of NF-κB/Rel in angelan-stimulated macrophages. Immunopharmacology 43, 1-9]. Angelan stimulates murine macrophage to produce cytokines including iNOS and activate NF-κB/Rel. In the present study, we investigated the role of CD14 and complement receptor type 3 (CR3) in mediating NO production and NF- κB/Rel activation induced by angelan and LPS. Three major differences between angelan and LPS were observed. First, angelan does not require serum proteins for NO response and NF-κB/Rel activation, while the activation by LPS requires serum proteins. Second, blocking of either CD14 or CR3 decreased angelan-induced NO response, while LPS-mediated NO production was inhibited by anti-CD14 mAb only. Third, angelan induced strong NF-κB/Rel and slight AP-1 DNA binding, whereas LPS potently activated both NF-κB/Rel and AP-1. Both angelan and LPS degraded IκB proteins and subsequently induced the mobilization of NF-κB/Rel proteins (p65, c-rel and p50) into nucleus. This suggests that macrophages display a common signaling machinery leading to the NF-κB/Rel activation in response to different stimulants. In conclusion, angelan and LPS use the membrane receptor CD14 and CR3 differentially for signaling NF-κB/Rel activation and NO production. (C) 2000 Elsevier Science B.V.
KW - Angelan
KW - Angelica gigas Nakai
KW - CD14
KW - CR3
KW - Macrophages
KW - NF-κB/Rel
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U2 - 10.1016/S0162-3109(00)00243-5
DO - 10.1016/S0162-3109(00)00243-5
M3 - Article
C2 - 10996025
AN - SCOPUS:0033829348
SN - 0162-3109
VL - 49
SP - 275
EP - 284
JO - Immunopharmacology
JF - Immunopharmacology
IS - 3
ER -