Differential effects of clathrin and actin inhibitors on internalization of Escherichia coli and Salmonella choleraesuis in porcine jejunal Peyer's patches

Benedict T. Green, David R. Brown

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Peyer's patches constitute both an inductive immune site and an enteropathogen invasion route. Peyer's patch mucosae from porcine jejunum were mounted in Ussing chambers, and either Salmonella choleraesuis vaccine strain SC-54 or non-pathogenic rodent and porcine Escherichia coli strains contacted the Peyer's patch mucosa for 90 min. Internalized bacteria were quantified by a gentamicin resistance assay. Monodansylcadaverine (300 μM, luminal addition), an inhibitor of clathrin-mediated endocytosis, significantly inhibited internalization of both E. coli strains relative to tissues untreated with the inhibitor; internalization of SC-54 was unaffected. The actin-disrupting agent cytochalasin D (10 μM, luminal addition), inhibited internalization of pig-adapted E. coli but not that of rodent-adapted E. coli or SC-54. Internalization of SC-54 and non-pathogenic E. coli in Peyer's patches appears to occur through different cellular routes.

Original languageEnglish (US)
Pages (from-to)117-122
Number of pages6
JournalVeterinary Microbiology
Volume113
Issue number1-2
DOIs
StatePublished - Mar 10 2006

Bibliographical note

Funding Information:
This work was supported in part by National Institutes of Health grants R01 DA-10200 and T32 DA-007239. The authors thank Dr. Carol L. Wells (Department of Laboratory Medicine and Pathology, University of Minnesota) for generously providing the M-21 strain of E. coli for this investigation, Dr. Sanford Weisberg (University of Minnesota Statistical Consulting Service) for expert advice on data analysis and Lisa Price for excellent technical assistance.

Keywords

  • Clathrin
  • Cytochalasin D
  • Endocytosis
  • Enteropathogen
  • Escherichia coli
  • Intracellular internalization
  • Monodansylcadaverine
  • Mucosal immunity
  • Salmonella choleraesuis

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