Differential host cell gene expression regulated by the porcine reproductive and respiratory syndrome virus GP4 and GP5 glycoproteins

Changhee Lee, Aimee Bachand, Michael P. Murtaugh, Dongwan Yoo

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The porcine reproductive and respiratory syndrome virus (PRRSV) GP4 and GP5 proteins are two membrane-associated viral glycoproteins that have been shown to induce neutralizing antibodies. In the present study, the host cell gene expression profiles altered by the GP4 and GP5 proteins were investigated by the use of DNA microarrays. Sublines of Marc-145 and HeLa cells were established by stable transfection with open reading frame (ORF)4 and ORF5 of PRRSV, respectively, and differential gene expressions were studied using microarray chips embedded with 1718 human-expressed sequence tags. The genes for protein degradation, protein synthesis and transport, and various other biochemical pathways were identified. No genes involved in the apoptosis pathway appeared to be regulated in GP5-expressing cells. The microarray data may provide insights into the specific cellular responses to the GP4 and GP5 proteins during PRRSV infection.

Original languageEnglish (US)
Pages (from-to)189-198
Number of pages10
JournalVeterinary immunology and immunopathology
Volume102
Issue number3
DOIs
StatePublished - Dec 8 2004

Bibliographical note

Funding Information:
The authors are grateful to Ryan Dowling for his technical assistance for microarray analysis. This study was supported by NSERC, Ontario Pork, the Ontario Ministry of Agriculture and Food (Animal Program).

Keywords

  • GP4 protein
  • GP5 protein
  • Gene regulation
  • Microarray
  • PRRS

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