Differentiating short- and long-term effects of diet in the obese mouse using 1H-nuclear magnetic resonance metabolomics

G. E. Duggan, D. S. Hittel, C. C. Hughey, A. Weljie, H. J. Vogel, J. Shearer

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

This study determined whether targeted metabolomic profiling of serum, using 1H nuclear magnetic resonance, could be employed to distinguish the effects of obesity from those of diet in mice. Following weaning, littermates were randomly divided into two diet groups: chow and high fat. After 12 weeks of dietary manipulation, fat-fed animals were obese and hyperglycaemic. Mice from each treatment either maintained their current diet or switched to the opposite diet for a final week. Differences in metabolite levels were determined using orthogonal projection to latent structures and cross-validated discriminant analysis. The short- and long-term effects of each diet could be clearly distinguished. Short-term diet effects are the major contributor to the metabolic profile, underscoring the need for controls beyond the standard fast before serum collection. This work shows the importance of dietary controls when attempting to isolate obesity-related changes and highlights the ability of metabolomics to identify subtle changes when experiments are properly structured.

Original languageEnglish (US)
Pages (from-to)859-862
Number of pages4
JournalDiabetes, Obesity and Metabolism
Volume13
Issue number9
DOIs
StatePublished - Sep 2011
Externally publishedYes

Keywords

  • Control
  • Design
  • Diet
  • Metabolism
  • NMR
  • Obesity

Fingerprint

Dive into the research topics of 'Differentiating short- and long-term effects of diet in the obese mouse using <sup>1</sup>H-nuclear magnetic resonance metabolomics'. Together they form a unique fingerprint.

Cite this