Differentiation of epidermal keratinocytes from human embryonic stem cells

Fahad K. Kidwai, Tong Cao, Kai Lu

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

For many years, cell therapies have been hampered by limited availability and inter-batch variability of primary cells. Human embryonic stem cell (hESC) can give rise to specialized cells like keratinocytes and recently emerged as a virtually unlimited source of potential therapeutic cells. However, xenogeneic components in differentiation cocktails have been limiting the clinical potential of hESC-derived keratinocytes (hESCs-Kert). Here, we demonstrated efficient differentiation of H9 human embryonic stem cells (H9-hESCs) into keratinocytes (H9-KertACC) in an autogenic co-culture system. We used activin as the main factor to induce keratinocyte differentiation. H9-Kert ACC expressed keratinocyte markers at mRNA and protein levels. Establishment of such animal-free microenvironment for keratinocyte differentiation will accelerate potential clinical application of hESCs.

Original languageEnglish (US)
Title of host publicationEpidermal Cells
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages13-22
Number of pages10
ISBN (Print)9781493912230
DOIs
StatePublished - Jan 1 2014

Publication series

NameMethods in Molecular Biology
Volume1195
ISSN (Print)1064-3745

Keywords

  • Activin
  • Co-culture
  • Differentiation
  • Epidermal
  • Human embryonic stem cell
  • Keratinocyte
  • Tissue engineering
  • Xeno-free

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