@inbook{ee848b1dab604bce8bfa8e9d84887608,
title = "Differentiation of epidermal keratinocytes from human embryonic stem cells",
abstract = "For many years, cell therapies have been hampered by limited availability and inter-batch variability of primary cells. Human embryonic stem cell (hESC) can give rise to specialized cells like keratinocytes and recently emerged as a virtually unlimited source of potential therapeutic cells. However, xenogeneic components in differentiation cocktails have been limiting the clinical potential of hESC-derived keratinocytes (hESCs-Kert). Here, we demonstrated efficient differentiation of H9 human embryonic stem cells (H9-hESCs) into keratinocytes (H9-KertACC) in an autogenic co-culture system. We used activin as the main factor to induce keratinocyte differentiation. H9-Kert ACC expressed keratinocyte markers at mRNA and protein levels. Establishment of such animal-free microenvironment for keratinocyte differentiation will accelerate potential clinical application of hESCs.",
keywords = "Activin, Co-culture, Differentiation, Epidermal, Human embryonic stem cell, Keratinocyte, Tissue engineering, Xeno-free",
author = "Kidwai, {Fahad K.} and Tong Cao and Kai Lu",
year = "2014",
month = jan,
day = "1",
doi = "10.1007/7651-2013-46",
language = "English (US)",
isbn = "9781493912230",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "13--22",
booktitle = "Epidermal Cells",
}