TY - GEN
T1 - Differentiation of Pluripotent Stem Cells on Multiwalled Carbon Nanotubes
AU - Holy, Jon
AU - Perkins, Edward
AU - Yu, Xun
PY - 2009
Y1 - 2009
N2 - This paper studies the adhesion, growth, and differentiation of stem cells on carbon nanotube matrices. Glass coverslips were coated with multiwalled carbon nanotube (MWNT) thin films using layer-by-layer self-assembling techniques. Pluripotent P19 mouse embryonal carcinoma stem cells were seeded onto uncoated or MWNT-coated coverslips, and either maintained in an undifferentiated state or induced to differentiate by the addition of retinoic acid. We found that cell adhesion was increased on the MWNT-coated surfaces, and that the expression patterns of some differentiation markers were altered in cells grown on MWNTs. The results suggest that MWNTs will be useful in directing pluripotent stem cell differentiation for tissue engineering purposes.
AB - This paper studies the adhesion, growth, and differentiation of stem cells on carbon nanotube matrices. Glass coverslips were coated with multiwalled carbon nanotube (MWNT) thin films using layer-by-layer self-assembling techniques. Pluripotent P19 mouse embryonal carcinoma stem cells were seeded onto uncoated or MWNT-coated coverslips, and either maintained in an undifferentiated state or induced to differentiate by the addition of retinoic acid. We found that cell adhesion was increased on the MWNT-coated surfaces, and that the expression patterns of some differentiation markers were altered in cells grown on MWNTs. The results suggest that MWNTs will be useful in directing pluripotent stem cell differentiation for tissue engineering purposes.
UR - http://www.scopus.com/inward/record.url?scp=77950972217&partnerID=8YFLogxK
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U2 - 10.1109/IEMBS.2009.5333699
DO - 10.1109/IEMBS.2009.5333699
M3 - Conference contribution
C2 - 19964415
AN - SCOPUS:77950972217
SN - 9781424432967
T3 - Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009
SP - 6022
EP - 6025
BT - Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009
PB - IEEE Computer Society
T2 - 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009
Y2 - 2 September 2009 through 6 September 2009
ER -