Nasopharyngeal carcinoma (NPC) has a high metastatic clinicopathological feature. As a carcinogen factor, N,N′-dinitrosopiperazine (DNP) is involved in NPC metastasis, but its precise mechanism has not been fully elucidated. Herein, we showed that DNP promotes NPC metastasis through upregulating miR-149. DNP was found to decrease Plakophilin3 (PKP3) expression, further DNP-decreased PKP3 was verified to be through upregulating miR-149. We also found that DNP induced proliferation, adhesion, migration and invasion of NPC cell, which was inhibited by miR-149-inhibitor. DNP may promote NPC metastasis through miR-149-decreased PKP3 expression. Therefore, DNP-increased miR-149 expression may be an important factor of NPC high metastasis, and miR-149 may serve as a molecular target for anti-metastasis therapy of NPC.
Bibliographical noteFunding Information:
We thank Dr Daofa Tian and Prof Tieying Li for kindly providing DNP. We appreciate the contributions and helpful discussion of various members in Clinical Laboratory of Zhuhai Hospital, Jinan University. And This work was supported in part by the National Natural Science Foundation of China (81372282, 81872226, 81402368, 81402265, 81502346), Guangdong Provincial Science and Technology Project Foundation (2017A020215136), Guangdong Natural Science Foundation (S2013010013360), China Postdoctoral Science Foundation funded project (2016M592580).
National Natural Science Foundation of China, Grant numbers: 81372282, 81402265, 81402368, 81502346, 81872226
© 2018 The Authors. Molecular Carcinogenesis Published by Wiley Periodicals, Inc.
- nasopharyngeal carcinoma