Diphenylene iodonium blocks K+ and Ca2+ currents in type I cells isolated from the neonatal rat carotid body

C. N. Wyatt, Edward K Weir, C. Peers

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Diphenylene iodonium (DPI), an inhibitor of NAD(P)H oxidase, blocks hypoxic excitation of the carotid body. We use the whole-cell patch-clamp technique to investigate the actions of DPI on ionic currents in isolated type I carotid body cells. DPI (10 μM) caused reversible blockade of K+ and Ca2+ currents in these cells, indicating that DPI is a non-selective ion channel blocker. Since hypoxic excitation of the carotid body is dependent on Ca2+ influx into type I cells, our observation that DPI blocks Ca2+ currents in type I cells can account for the ability of this compound to inhibit hypoxic excitation of the intact organ.

Original languageEnglish (US)
Pages (from-to)63-66
Number of pages4
JournalNeuroscience Letters
Volume172
Issue number1-2
DOIs
StatePublished - May 19 1994

Keywords

  • Ca current
  • Carotid body
  • Chemoreceptor
  • Diphenylene iodonium
  • K current
  • Type I cell

Fingerprint Dive into the research topics of 'Diphenylene iodonium blocks K<sup>+</sup> and Ca<sup>2+</sup> currents in type I cells isolated from the neonatal rat carotid body'. Together they form a unique fingerprint.

Cite this