Abstract
An X ray fluorescence method for measurement of total serum phospholipid is described. A Folch extract of 1 ml of serum was evaporated and deposited on a paper disc by washing with chloroform. A coefficient of variation of 4% was obtained for samples containing at least 1 micromole of lipid, as phosphorus. The major advantage of the method is its speed (5 min/sample) in comparison to the phosphomolybdenum blue procedure. The method is free of interference from other lipid constituents and the phosphorous content can be standardized by comparison with very stable inorganic phosphates.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1271-1274 |
| Number of pages | 4 |
| Journal | Clinical chemistry |
| Volume | 20 |
| Issue number | 10 |
| DOIs | |
| State | Published - 1974 |