Direct fluorescence monitoring of DNA base excision repair

Toshikazu Ono; Shenliang Wang; Chi Kin Koo; Lisa Engstrom; Sheila S. David; Eric T. Kool

doi:10.1002/anie.201108135

Direct fluorescence monitoring of DNA base excision repair

Toshikazu Ono, Shenliang Wang, Chi Kin Koo, Lisa Engstrom, Sheila S. David, Eric T. Kool

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72 Scopus citations

Abstract

Uracil is a common form of DNA damage resulting from hydrolysis of cytosine, and cellular uracil DNA glycosylases (UDG) have evolved to remove it specifically. The use of nonnatural pyrene deoxyriboside in short DNA oligomers to directly report on UDG enzymatic activity is described. The mechanism relies on the use of uracil as a strong quencher of pyrene, and enzyme repair activity can be directly imaged with bacterial cells in real time.

Original language	English (US)
Pages (from-to)	1689-1692
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	51
Issue number	7
DOIs	https://doi.org/10.1002/anie.201108135
State	Published - Feb 13 2012
Externally published	Yes

Keywords

fluorescence
oligonucleotides
pyrene
uracil
uracil DNA glycosylase

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title = "Direct fluorescence monitoring of DNA base excision repair",

abstract = "Uracil is a common form of DNA damage resulting from hydrolysis of cytosine, and cellular uracil DNA glycosylases (UDG) have evolved to remove it specifically. The use of nonnatural pyrene deoxyriboside in short DNA oligomers to directly report on UDG enzymatic activity is described. The mechanism relies on the use of uracil as a strong quencher of pyrene, and enzyme repair activity can be directly imaged with bacterial cells in real time.",

keywords = "fluorescence, oligonucleotides, pyrene, uracil, uracil DNA glycosylase",

author = "Toshikazu Ono and Shenliang Wang and Koo, {Chi Kin} and Lisa Engstrom and David, {Sheila S.} and Kool, {Eric T.}",

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doi = "10.1002/anie.201108135",

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volume = "51",

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AU - Ono, Toshikazu

AU - Wang, Shenliang

AU - Koo, Chi Kin

AU - Engstrom, Lisa

AU - David, Sheila S.

AU - Kool, Eric T.

PY - 2012/2/13

Y1 - 2012/2/13

N2 - Uracil is a common form of DNA damage resulting from hydrolysis of cytosine, and cellular uracil DNA glycosylases (UDG) have evolved to remove it specifically. The use of nonnatural pyrene deoxyriboside in short DNA oligomers to directly report on UDG enzymatic activity is described. The mechanism relies on the use of uracil as a strong quencher of pyrene, and enzyme repair activity can be directly imaged with bacterial cells in real time.

AB - Uracil is a common form of DNA damage resulting from hydrolysis of cytosine, and cellular uracil DNA glycosylases (UDG) have evolved to remove it specifically. The use of nonnatural pyrene deoxyriboside in short DNA oligomers to directly report on UDG enzymatic activity is described. The mechanism relies on the use of uracil as a strong quencher of pyrene, and enzyme repair activity can be directly imaged with bacterial cells in real time.

KW - fluorescence

KW - oligonucleotides

KW - pyrene

KW - uracil

KW - uracil DNA glycosylase

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

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ER -

Direct fluorescence monitoring of DNA base excision repair

Abstract

Keywords

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