Direct measurements of the coordination of lever arm swing and the catalytic cycle in myosin V

Darshan V. Trivedia, Joseph M. Murettab, Anja M. Swensona, Jonathon P. Davisc, David D. Thomasb, Christopher M. Yengoa

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Myosins use a conserved structural mechanism to convert the energy from ATP hydrolysis into a large swing of the force-generating lever arm. The precise timing of the lever arm movement with respect to the steps in the actomyosin ATPase cycle has not been determined. We have developed a FRET system in myosin V that uses three donor-acceptor pairs to examine the kinetics of lever arm swing during the recovery and power stroke phases of the ATPase cycle. During the recovery stroke the lever arm swing is tightly coupled to priming the active site for ATP hydrolysis. The lever arm swing during the power stroke occurs in two steps, a fast step that occurs before phosphate release and a slow step that occurs before ADP release. Time-resolved FRET demonstrates a 20-Å change in distance between the pre- and postpower stroke states and shows that the lever arm is more dynamic in the postpower stroke state. Our results suggest myosin binding to actin in the ADP.Pi complex triggers a rapid power stroke that gates the release of phosphate, whereas a second slower power stroke may be important for mediating strain sensitivity.

Original languageEnglish (US)
Pages (from-to)14593-14598
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number47
StatePublished - Nov 24 2015


  • FRET
  • Force generation
  • Kinetics
  • Myosin|actin

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