Directed evolution of Escherichia coli farnesyl diphosphate synthase (IspA) reveals novel structural determinants of chain length specificity

Engineering & Materials Science

• Carotenoids 95%
• Chain length 80%
• Escherichia coli 72%
• Enzymes 43%
• Elongation 35%
• Molecular modeling 28%
• Assays 22%
• Substitution reactions 18%
• Color 13%
• Substrates 13%

Medicine & Life Sciences

• Geranyltranstransferase 100%
• Escherichia coli 40%
• Aspartic Acid 40%
• Carotenoids 34%
• Mutation 32%
• prenyl 31%
• Clone Cells 30%
• geranylgeranyl pyrophosphate 15%
• farnesyl pyrophosphate 14%
• Farnesyltranstransferase 14%
• Amino Acids 13%
• Diphosphates 12%
• Enzymes 10%
• Color 7%
• Polymerase Chain Reaction 5%
• In Vitro Techniques 4%

Chemical Compounds

• Farnesyl Diphosphate 86%
• Mutation 48%
• Aspartate 42%
• Length 34%
• Carotenoid 31%
• Prenyl Diphosphate 17%
• Geranylgeranyl Diphosphate 15%
• Product Distribution 9%
• Molecular Modeling 8%
• Acid 6%
• Substitution Reaction 6%