Discovery of folds, knots, and S-folds in long molecules of DNA stretched in nanochannels

Jeffrey G. Reifenberger, Kevin D. Dorfman, Han Cao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

BioNano Genomics has developed a method to fluorescently label long molecules of DNA, typically upwards of 100 kilobases, at nick sites generating a unique barcode pattern that can either be mapped to a reference or de novo assembled. In the present work, we demonstrate the ability to detect topological events in confined DNA from E. coli through identifying anomalous bright regions in the standard inter-calator YOYO signal along the length of the molecule. Topological events in DNA can mimic genomic structural variations by generating regions that appear as deletions or insertions to the reference resulting in false calls.

Original languageEnglish (US)
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages326-328
Number of pages3
ISBN (Electronic)9780979806483
StatePublished - Jan 1 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: Oct 25 2015Oct 29 2015

Publication series

NameMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period10/25/1510/29/15

Keywords

  • Backfolds
  • DNA
  • Folds
  • Genomic mapping
  • Knots
  • Nanochannels
  • Topological events

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