Abstract
BioNano Genomics has developed a method to fluorescently label long molecules of DNA, typically upwards of 100 kilobases, at nick sites generating a unique barcode pattern that can either be mapped to a reference or de novo assembled. In the present work, we demonstrate the ability to detect topological events in confined DNA from E. coli through identifying anomalous bright regions in the standard inter-calator YOYO signal along the length of the molecule. Topological events in DNA can mimic genomic structural variations by generating regions that appear as deletions or insertions to the reference resulting in false calls.
| Original language | English (US) |
|---|---|
| Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 326-328 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| State | Published - 2015 |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: Oct 25 2015 → Oct 29 2015 |
Publication series
| Name | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Other
| Other | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
|---|---|
| Country/Territory | Korea, Republic of |
| City | Gyeongju |
| Period | 10/25/15 → 10/29/15 |
Bibliographical note
Funding Information:This Work was supported by the NIH (R01-HG006851). Jeffrey G. Reifenberger and Han Cao are employees of BioNano Genomics, which sells the Iyrs platform for genomic mapping.
Publisher Copyright:
© 15CBMS-0001.
Keywords
- Backfolds
- DNA
- Folds
- Genomic mapping
- Knots
- Nanochannels
- Topological events