Dissecting plant chromosomes by the use of ionizing radiation

Penny M.A. Kianian; Katie L. Liberatore; Marisa E. Miller; Justin B. Hegstad; Shahryar F. Kianian

doi:10.1007/978-1-4939-3622-9_8

Dissecting plant chromosomes by the use of ionizing radiation

Penny M.A. Kianian, Katie L. Liberatore, Marisa E. Miller, Justin B. Hegstad, Shahryar F. Kianian

Research output: Contribution to journal › Article › peer-review

Abstract

Radiation treatment of genomes is used to generate chromosome breaks for numerous applications. This protocol describes the preparation of seeds and the determination of the optimal level of irradiation dosage for the creation of a radiation hybrid (RH) population. These RH lines can be used to generate high- resolution physical maps for the assembly of sequenced genomes as well as the fine mapping of genes. This procedure can also be used for mutation breeding and forward/reverse genetics.

Original language	English (US)
Pages (from-to)	91-101
Number of pages	11
Journal	Methods in Molecular Biology
Volume	1429
DOIs	https://doi.org/10.1007/978-1-4939-3622-9_8
State	Published - Jan 1 2016

Keywords

Gamma rays
Gene cloning
Mapping
Mutation
Radiation hybrid
Tempering seed

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AU - Kianian, Shahryar F.

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AB - Radiation treatment of genomes is used to generate chromosome breaks for numerous applications. This protocol describes the preparation of seeds and the determination of the optimal level of irradiation dosage for the creation of a radiation hybrid (RH) population. These RH lines can be used to generate high- resolution physical maps for the assembly of sequenced genomes as well as the fine mapping of genes. This procedure can also be used for mutation breeding and forward/reverse genetics.

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KW - Tempering seed

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Dissecting plant chromosomes by the use of ionizing radiation

Abstract

Keywords

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