Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae)

Michaela Horčičková; Šárka Čondlová; Nikola Holubová; Bohumil Sak; Dana Květoåová; Lenka Hlásková; Roman Konečný; František Sedláček; Mark Clark; Catherine Giddings; John McEvoy; Martin Kváč

doi:10.1017/S0031182018001142

Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae)

Michaela Horčičková, Šárka Čondlová, Nikola Holubová, Bohumil Sak, Dana Květoåová, Lenka Hlásková, Roman Konečný, František Sedláček, Mark Clark, Catherine Giddings, John McEvoy, Martin Kváč

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Fecal samples from wild-caught common voles (n = 328) from 16 locations in the Czech Republic were screened for Cryptosporidium by microscopy and PCR/sequencing at loci coding small-subunit rRNA, Cryptosporidium oocyst wall protein, actin and 70 kDa heat shock protein. Cryptosporidium infections were detected in 74 voles (22.6%). Rates of infection did not differ between males and females nor between juveniles and adults. Phylogenetic analysis revealed the presence of eight Cryptosporidium species/genotypes including two new species, C. alticolis and C. microti. These species from wild-caught common voles were able to infect common and meadow voles under experimental conditions, with a prepatent period of 3-5 days post-infection (DPI), but they were not infectious for various other rodents or chickens. Meadow voles lost infection earlier than common voles (11-14 vs 13-16 DPI) and had significantly lower infection intensity. Cryptosporidium alticolis infects the anterior small intestine and has larger oocysts (5.4 × 4.9 μm), whereas C. microti infects the large intestine and has smaller oocysts (4.3 × 4.1 m). None of the rodents developed clinical signs of infection. Genetic and biological data support the establishment of C. alticolis and C. microti as separate species of the genus Cryptosporidium.

Original language	English (US)
Pages (from-to)	220-233
Number of pages	14
Journal	Parasitology
Volume	146
Issue number	2
DOIs	https://doi.org/10.1017/S0031182018001142
State	Published - Feb 1 2019
Externally published	Yes

Bibliographical note

Funding Information:
This study was funded by Ministry of Education, Youth and Sports of the Czech Republic (LTAUSA17165), the US National Institute of Allergy and Infectious Diseases (1R15AI122152-01A1), Grant Agency of University of South Bohemia (098/2016/Z and 002/2016/Z) and supported by MEYS CR (LM2015062 Czech-BioImaging). We thank Annaliese Beery, Jennifer Christensen, Nancy Owen and Karen Swiecanski of Smith College for providing meadow voles and for assistance with transporting voles to North Dakota State University for the study.

Publisher Copyright:
Copyright © 2018 Cambridge University Press.

Keywords

Experimental infection
Rodentia
molecular analyses
oocyst size
phylogeny
voles

Access

10.1017/S0031182018001142

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994189

OpenUrl availability

Full text

Cite this

Horčičková, M., Čondlová, Š., Holubová, N., Sak, B., Květoåová, D., Hlásková, L., Konečný, R., Sedláček, F., Clark, M., Giddings, C., McEvoy, J., & Kváč, M. (2019). Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae). Parasitology, 146(2), 220-233. https://doi.org/10.1017/S0031182018001142

Horčičková, M, Čondlová, Š, Holubová, N, Sak, B, Květoåová, D, Hlásková, L, Konečný, R, Sedláček, F, Clark, M, Giddings, C, McEvoy, J & Kváč, M 2019, 'Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae)', Parasitology, vol. 146, no. 2, pp. 220-233. https://doi.org/10.1017/S0031182018001142

@article{4d4985c4670948c899aa3834bc72dac7,

title = "Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae)",

abstract = "Fecal samples from wild-caught common voles (n = 328) from 16 locations in the Czech Republic were screened for Cryptosporidium by microscopy and PCR/sequencing at loci coding small-subunit rRNA, Cryptosporidium oocyst wall protein, actin and 70 kDa heat shock protein. Cryptosporidium infections were detected in 74 voles (22.6%). Rates of infection did not differ between males and females nor between juveniles and adults. Phylogenetic analysis revealed the presence of eight Cryptosporidium species/genotypes including two new species, C. alticolis and C. microti. These species from wild-caught common voles were able to infect common and meadow voles under experimental conditions, with a prepatent period of 3-5 days post-infection (DPI), but they were not infectious for various other rodents or chickens. Meadow voles lost infection earlier than common voles (11-14 vs 13-16 DPI) and had significantly lower infection intensity. Cryptosporidium alticolis infects the anterior small intestine and has larger oocysts (5.4 × 4.9 μm), whereas C. microti infects the large intestine and has smaller oocysts (4.3 × 4.1 m). None of the rodents developed clinical signs of infection. Genetic and biological data support the establishment of C. alticolis and C. microti as separate species of the genus Cryptosporidium.",

keywords = "Experimental infection, Rodentia, molecular analyses, oocyst size, phylogeny, voles",

author = "Michaela Hor{\v c}i{\v c}kov{\'a} and {\v S}{\'a}rka {\v C}ondlov{\'a} and Nikola Holubov{\'a} and Bohumil Sak and Dana Kv{\v e}to{\aa}ov{\'a} and Lenka Hl{\'a}skov{\'a} and Roman Kone{\v c}n{\'y} and Franti{\v s}ek Sedl{\'a}{\v c}ek and Mark Clark and Catherine Giddings and John McEvoy and Martin Kv{\'a}{\v c}",

note = "Funding Information: This study was funded by Ministry of Education, Youth and Sports of the Czech Republic (LTAUSA17165), the US National Institute of Allergy and Infectious Diseases (1R15AI122152-01A1), Grant Agency of University of South Bohemia (098/2016/Z and 002/2016/Z) and supported by MEYS CR (LM2015062 Czech-BioImaging). We thank Annaliese Beery, Jennifer Christensen, Nancy Owen and Karen Swiecanski of Smith College for providing meadow voles and for assistance with transporting voles to North Dakota State University for the study. Publisher Copyright: Copyright {\textcopyright} 2018 Cambridge University Press.",

year = "2019",

month = feb,

day = "1",

doi = "10.1017/S0031182018001142",

language = "English (US)",

volume = "146",

pages = "220--233",

journal = "Parasitology",

issn = "0031-1820",

publisher = "Cambridge University Press",

number = "2",

}

TY - JOUR

T1 - Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae)

AU - Horčičková, Michaela

AU - Čondlová, Šárka

AU - Holubová, Nikola

AU - Sak, Bohumil

AU - Květoåová, Dana

AU - Hlásková, Lenka

AU - Konečný, Roman

AU - Sedláček, František

AU - Clark, Mark

AU - Giddings, Catherine

AU - McEvoy, John

AU - Kváč, Martin

N1 - Funding Information: This study was funded by Ministry of Education, Youth and Sports of the Czech Republic (LTAUSA17165), the US National Institute of Allergy and Infectious Diseases (1R15AI122152-01A1), Grant Agency of University of South Bohemia (098/2016/Z and 002/2016/Z) and supported by MEYS CR (LM2015062 Czech-BioImaging). We thank Annaliese Beery, Jennifer Christensen, Nancy Owen and Karen Swiecanski of Smith College for providing meadow voles and for assistance with transporting voles to North Dakota State University for the study. Publisher Copyright: Copyright © 2018 Cambridge University Press.

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Fecal samples from wild-caught common voles (n = 328) from 16 locations in the Czech Republic were screened for Cryptosporidium by microscopy and PCR/sequencing at loci coding small-subunit rRNA, Cryptosporidium oocyst wall protein, actin and 70 kDa heat shock protein. Cryptosporidium infections were detected in 74 voles (22.6%). Rates of infection did not differ between males and females nor between juveniles and adults. Phylogenetic analysis revealed the presence of eight Cryptosporidium species/genotypes including two new species, C. alticolis and C. microti. These species from wild-caught common voles were able to infect common and meadow voles under experimental conditions, with a prepatent period of 3-5 days post-infection (DPI), but they were not infectious for various other rodents or chickens. Meadow voles lost infection earlier than common voles (11-14 vs 13-16 DPI) and had significantly lower infection intensity. Cryptosporidium alticolis infects the anterior small intestine and has larger oocysts (5.4 × 4.9 μm), whereas C. microti infects the large intestine and has smaller oocysts (4.3 × 4.1 m). None of the rodents developed clinical signs of infection. Genetic and biological data support the establishment of C. alticolis and C. microti as separate species of the genus Cryptosporidium.

AB - Fecal samples from wild-caught common voles (n = 328) from 16 locations in the Czech Republic were screened for Cryptosporidium by microscopy and PCR/sequencing at loci coding small-subunit rRNA, Cryptosporidium oocyst wall protein, actin and 70 kDa heat shock protein. Cryptosporidium infections were detected in 74 voles (22.6%). Rates of infection did not differ between males and females nor between juveniles and adults. Phylogenetic analysis revealed the presence of eight Cryptosporidium species/genotypes including two new species, C. alticolis and C. microti. These species from wild-caught common voles were able to infect common and meadow voles under experimental conditions, with a prepatent period of 3-5 days post-infection (DPI), but they were not infectious for various other rodents or chickens. Meadow voles lost infection earlier than common voles (11-14 vs 13-16 DPI) and had significantly lower infection intensity. Cryptosporidium alticolis infects the anterior small intestine and has larger oocysts (5.4 × 4.9 μm), whereas C. microti infects the large intestine and has smaller oocysts (4.3 × 4.1 m). None of the rodents developed clinical signs of infection. Genetic and biological data support the establishment of C. alticolis and C. microti as separate species of the genus Cryptosporidium.

KW - Experimental infection

KW - Rodentia

KW - molecular analyses

KW - oocyst size

KW - phylogeny

KW - voles

UR - http://www.scopus.com/inward/record.url?scp=85049975640&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049975640&partnerID=8YFLogxK

U2 - 10.1017/S0031182018001142

DO - 10.1017/S0031182018001142

M3 - Article

C2 - 30012231

AN - SCOPUS:85049975640

SN - 0031-1820

VL - 146

SP - 220

EP - 233

JO - Parasitology

JF - Parasitology

IS - 2

ER -

Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae)

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this