Dj1 deficiency protects against atherosclerosis with anti-inflammatory response in macrophages

Tharini Sivasubramaniyam, Jiaqi Yang, Henry S. Cheng, Alexandra Zyla, Angela Li, Rickvinder Besla, Idit Dotan, Xavier S. Revelo, Sally Yu Shi, Helen Le, Stephanie A. Schroer, David W. Dodington, Yoo Jin Park, Min Jeong Kim, Daniella Febbraro, Isabelle Ruel, Jacques Genest, Raymond H. Kim, Tak W. Mak, Daniel A. WinerClinton S. Robbins, Minna Woo

Research output: Contribution to journalArticlepeer-review

Abstract

Inflammation is a key contributor to atherosclerosis with macrophages playing a pivotal role through the induction of oxidative stress and cytokine/chemokine secretion. DJ1, an anti-oxidant protein, has shown to paradoxically protect against chronic and acute inflammation. However, the role of DJ1 in atherosclerosis remains elusive. To assess the role of Dj1 in atherogenesis, we generated whole-body Dj1-deficient atherosclerosis-prone Apoe null mice (Dj1−/−Apoe−/−). After 21 weeks of atherogenic diet, Dj1−/− Apoe−/−mice were protected against atherosclerosis with significantly reduced plaque macrophage content. To assess whether haematopoietic or parenchymal Dj1 contributed to atheroprotection in Dj1-deficient mice, we performed bone-marrow (BM) transplantation and show that Dj1-deficient BM contributed to their attenuation in atherosclerosis. To assess cell-autonomous role of macrophage Dj1 in atheroprotection, BM-derived macrophages from Dj1-deficient mice and Dj1-silenced macrophages were assessed in response to oxidized low-density lipoprotein (oxLDL). In both cases, there was an enhanced anti-inflammatory response which may have contributed to atheroprotection in Dj1-deficient mice. There was also an increased trend of plasma DJ-1 levels from individuals with ischemic heart disease compared to those without. Our findings indicate an atheropromoting role of Dj1 and suggests that targeting Dj1 may provide a novel therapeutic avenue for atherosclerosis treatment or prevention.

Original languageEnglish (US)
Article number4723
JournalScientific reports
Volume11
Issue number1
DOIs
StatePublished - Dec 2021
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by operating Grants from the Canadian Institute of Health Research (CIHR) MOP-142193 and PJT-159505, and the Heart and Stroke Foundation of Canada Grant-in-Aid to MW. MW is supported by the Canada Research Chair in Signal Transduction in Diabetes Pathogenesis and the Soham and Shaila Ajmera Family Chair in Molecular Diabetes Research. TS and SYS were supported by the CIHR Doctoral Research Award, Diabetes Canada Doctoral Student Research Award, the Canadian Liver Foundation Graduate Studentship, and the Banting and Best Diabetes Centre (BBDC) Novo Nordisk Studentship. AL was supported by the Heart & Stroke/Richard Lewar Centre of Excellence Studentship. YP was supported by fellowships from the CIHR and Diabetes Canada. DF was supported by the BBDC Novo Nordisk Studentship and Ontario Graduate Scholarship.

Publisher Copyright:
© 2021, The Author(s).

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