DNA gyrase and topoisomerase IV: Biochemical activities, physiological roles during chromosome replication, and drug sensitivities

Cindy Levine, Hiroshi Hiasa, Kenneth J. Marians

Research output: Contribution to journalReview articlepeer-review

291 Scopus citations

Abstract

DNA gyrase and topoisomerase IV are the two type II topoisomerases present in bacteria. Though clearly related, based on amino acid sequence similarity, they each play crucial, but distinct, roles in the cell. Gyrase is involved primarily in supporting nascent chain elongation during replication of the chromosome, whereas topoisomerase IV separates the topologically linked daughter chromosomes during the terminal stage of DNA replication. These different roles can be attributed to differences in the biochemical properties of the two enzymes. The biochemical activities, physiological roles, and drug sensitivities of the enzymes are reviewed.

Original languageEnglish (US)
Pages (from-to)29-43
Number of pages15
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1400
Issue number1-3
DOIs
StatePublished - Oct 1 1998
Externally publishedYes

Bibliographical note

Funding Information:
Studies from the author’s laboratory were supported by NIH Grant GM34558.

Keywords

  • Chromosome decatenation
  • DNA replication
  • Drug resistance
  • Mechanism
  • Type II

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