Dopamine metabolism by a monoamine oxidase mitochondrial shuttle activates the electron transport chain

Steven M. Graves; Zhong Xie; Kristen A. Stout; Enrico Zampese; Lena F. Burbulla; Jean C. Shih; Jyothisri Kondapalli; Tomasso Patriarchi; Lin Tian; Lars Brichta; Paul Greengard; Dimitri Krainc; Paul T. Schumacker; D. James Surmeier

doi:10.1038/s41593-019-0556-3

Dopamine metabolism by a monoamine oxidase mitochondrial shuttle activates the electron transport chain

Steven M. Graves, Zhong Xie, Kristen A. Stout, Enrico Zampese, Lena F. Burbulla, Jean C. Shih, Jyothisri Kondapalli, Tomasso Patriarchi, Lin Tian, Lars Brichta, Paul Greengard, Dimitri Krainc, Paul T. Schumacker, D. James Surmeier

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Monoamine oxidase (MAO) metabolizes cytosolic dopamine (DA), thereby limiting auto-oxidation, but is also thought to generate cytosolic hydrogen peroxide (H₂O₂). We show that MAO metabolism of DA does not increase cytosolic H₂O₂ but leads to mitochondrial electron transport chain (ETC) activity. This is dependent upon MAO anchoring to the outer mitochondrial membrane and shuttling electrons through the intermembrane space to support the bioenergetic demands of phasic DA release.

Original language	English (US)
Pages (from-to)	15-20
Number of pages	6
Journal	Nature neuroscience
Volume	23
Issue number	1
DOIs	https://doi.org/10.1038/s41593-019-0556-3
State	Published - Jan 1 2020
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by grants from the JPB Foundation, the IDP Foundation, the Michael J. Fox Foundation and the NIH (NS047085) to D.J.S.; an NIH grant (NS076054) to D.K.; an NIH grant (DA039253) and a Northwestern Memorial Foundation grant to S.M.G.; the Boyd and Elsi Welin Professorship and Tsai Family Fund to J.C.S.; and NIH grants U01NS103522, U01NS090604 and DPMH107056 to L.T. The authors thank the Northwestern Center for Advanced Microscopy (supported by National Cancer Center Support Grant P30 CA060553) for assistance.

PubMed: MeSH publication types

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Access

10.1038/s41593-019-0556-3

Fingerprint Dive into the research topics of 'Dopamine metabolism by a monoamine oxidase mitochondrial shuttle activates the electron transport chain'. Together they form a unique fingerprint.

Cite this

Graves, S. M., Xie, Z., Stout, K. A., Zampese, E., Burbulla, L. F., Shih, J. C., Kondapalli, J., Patriarchi, T., Tian, L., Brichta, L., Greengard, P., Krainc, D., Schumacker, P. T., & Surmeier, D. J. (2020). Dopamine metabolism by a monoamine oxidase mitochondrial shuttle activates the electron transport chain. Nature neuroscience, 23(1), 15-20. https://doi.org/10.1038/s41593-019-0556-3

Dopamine metabolism by a monoamine oxidase mitochondrial shuttle activates the electron transport chain. / Graves, Steven M.; Xie, Zhong; Stout, Kristen A.; Zampese, Enrico; Burbulla, Lena F.; Shih, Jean C.; Kondapalli, Jyothisri; Patriarchi, Tomasso; Tian, Lin; Brichta, Lars; Greengard, Paul; Krainc, Dimitri; Schumacker, Paul T.; Surmeier, D. James.

In: Nature neuroscience, Vol. 23, No. 1, 01.01.2020, p. 15-20.

Research output: Contribution to journal › Article › peer-review

Graves, SM, Xie, Z, Stout, KA, Zampese, E, Burbulla, LF, Shih, JC, Kondapalli, J, Patriarchi, T, Tian, L, Brichta, L, Greengard, P, Krainc, D, Schumacker, PT & Surmeier, DJ 2020, 'Dopamine metabolism by a monoamine oxidase mitochondrial shuttle activates the electron transport chain', Nature neuroscience, vol. 23, no. 1, pp. 15-20. https://doi.org/10.1038/s41593-019-0556-3

Graves, Steven M. ; Xie, Zhong ; Stout, Kristen A. ; Zampese, Enrico ; Burbulla, Lena F. ; Shih, Jean C. ; Kondapalli, Jyothisri ; Patriarchi, Tomasso ; Tian, Lin ; Brichta, Lars ; Greengard, Paul ; Krainc, Dimitri ; Schumacker, Paul T. ; Surmeier, D. James. / Dopamine metabolism by a monoamine oxidase mitochondrial shuttle activates the electron transport chain. In: Nature neuroscience. 2020 ; Vol. 23, No. 1. pp. 15-20.

@article{cbef33f9d1824341843cff0eb8c77be1,

title = "Dopamine metabolism by a monoamine oxidase mitochondrial shuttle activates the electron transport chain",

abstract = "Monoamine oxidase (MAO) metabolizes cytosolic dopamine (DA), thereby limiting auto-oxidation, but is also thought to generate cytosolic hydrogen peroxide (H2O2). We show that MAO metabolism of DA does not increase cytosolic H2O2 but leads to mitochondrial electron transport chain (ETC) activity. This is dependent upon MAO anchoring to the outer mitochondrial membrane and shuttling electrons through the intermembrane space to support the bioenergetic demands of phasic DA release.",

author = "Graves, {Steven M.} and Zhong Xie and Stout, {Kristen A.} and Enrico Zampese and Burbulla, {Lena F.} and Shih, {Jean C.} and Jyothisri Kondapalli and Tomasso Patriarchi and Lin Tian and Lars Brichta and Paul Greengard and Dimitri Krainc and Schumacker, {Paul T.} and Surmeier, {D. James}",

note = "Funding Information: This study was supported by grants from the JPB Foundation, the IDP Foundation, the Michael J. Fox Foundation and the NIH (NS047085) to D.J.S.; an NIH grant (NS076054) to D.K.; an NIH grant (DA039253) and a Northwestern Memorial Foundation grant to S.M.G.; the Boyd and Elsi Welin Professorship and Tsai Family Fund to J.C.S.; and NIH grants U01NS103522, U01NS090604 and DPMH107056 to L.T. The authors thank the Northwestern Center for Advanced Microscopy (supported by National Cancer Center Support Grant P30 CA060553) for assistance.",

year = "2020",

month = jan,

day = "1",

doi = "10.1038/s41593-019-0556-3",

language = "English (US)",

volume = "23",

pages = "15--20",

journal = "Nature Neuroscience",

issn = "1097-6256",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - Dopamine metabolism by a monoamine oxidase mitochondrial shuttle activates the electron transport chain

AU - Graves, Steven M.

AU - Xie, Zhong

AU - Stout, Kristen A.

AU - Zampese, Enrico

AU - Burbulla, Lena F.

AU - Shih, Jean C.

AU - Kondapalli, Jyothisri

AU - Patriarchi, Tomasso

AU - Tian, Lin

AU - Brichta, Lars

AU - Greengard, Paul

AU - Krainc, Dimitri

AU - Schumacker, Paul T.

AU - Surmeier, D. James

N1 - Funding Information: This study was supported by grants from the JPB Foundation, the IDP Foundation, the Michael J. Fox Foundation and the NIH (NS047085) to D.J.S.; an NIH grant (NS076054) to D.K.; an NIH grant (DA039253) and a Northwestern Memorial Foundation grant to S.M.G.; the Boyd and Elsi Welin Professorship and Tsai Family Fund to J.C.S.; and NIH grants U01NS103522, U01NS090604 and DPMH107056 to L.T. The authors thank the Northwestern Center for Advanced Microscopy (supported by National Cancer Center Support Grant P30 CA060553) for assistance.

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Monoamine oxidase (MAO) metabolizes cytosolic dopamine (DA), thereby limiting auto-oxidation, but is also thought to generate cytosolic hydrogen peroxide (H2O2). We show that MAO metabolism of DA does not increase cytosolic H2O2 but leads to mitochondrial electron transport chain (ETC) activity. This is dependent upon MAO anchoring to the outer mitochondrial membrane and shuttling electrons through the intermembrane space to support the bioenergetic demands of phasic DA release.

AB - Monoamine oxidase (MAO) metabolizes cytosolic dopamine (DA), thereby limiting auto-oxidation, but is also thought to generate cytosolic hydrogen peroxide (H2O2). We show that MAO metabolism of DA does not increase cytosolic H2O2 but leads to mitochondrial electron transport chain (ETC) activity. This is dependent upon MAO anchoring to the outer mitochondrial membrane and shuttling electrons through the intermembrane space to support the bioenergetic demands of phasic DA release.

UR - http://www.scopus.com/inward/record.url?scp=85076500453&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85076500453&partnerID=8YFLogxK

U2 - 10.1038/s41593-019-0556-3

DO - 10.1038/s41593-019-0556-3

M3 - Article

C2 - 31844313

AN - SCOPUS:85076500453

VL - 23

SP - 15

EP - 20

JO - Nature Neuroscience

JF - Nature Neuroscience

SN - 1097-6256

IS - 1

ER -