TY - JOUR
T1 - Double-cross hydrostatic pressure sample injection for chip CE
T2 - Variable sample plug volume and minimum number of electrodes
AU - Luo, Yong
AU - Wu, Dapeng
AU - Zeng, Shaojiang
AU - Gai, Hongwei
AU - Long, Zhicheng
AU - Shen, Zheng
AU - Dai, Zhongpeng
AU - Qin, Jianhua
AU - Lin, Bingcheng
PY - 2006/9/1
Y1 - 2006/9/1
N2 - A novel sample injection method for chip CE was presented. This injection method uses hydrostatic pressure, generated by emptying the sample waste reservoir, for sample loading and electrokinetic force for dispensing. The injection was performed on a double-cross microchip. One cross, created by the sample and separation channels, is used for formation of a sample plug. Another cross, formed by the sample and controlling channels, is used for plug control. By varying the electric field in the controlling channel, the sample plug volume can be linearly adjusted. Hydrostatic pressure takes advantage of its ease of generation on a microfluidic chip, without any electrode or external pressure pump, thus allowing a sample injection with a minimum number of electrodes. The potential of this injection method was demonstrated by a four-separation-channel chip CE system. In this system, parallel sample separation can be achieved with only two electrodes, which is otherwise impossible with conventional injection methods. Hydrostatic pressure maintains the sample composition during the sample loading, allowing the injection to be free of injection bias.
AB - A novel sample injection method for chip CE was presented. This injection method uses hydrostatic pressure, generated by emptying the sample waste reservoir, for sample loading and electrokinetic force for dispensing. The injection was performed on a double-cross microchip. One cross, created by the sample and separation channels, is used for formation of a sample plug. Another cross, formed by the sample and controlling channels, is used for plug control. By varying the electric field in the controlling channel, the sample plug volume can be linearly adjusted. Hydrostatic pressure takes advantage of its ease of generation on a microfluidic chip, without any electrode or external pressure pump, thus allowing a sample injection with a minimum number of electrodes. The potential of this injection method was demonstrated by a four-separation-channel chip CE system. In this system, parallel sample separation can be achieved with only two electrodes, which is otherwise impossible with conventional injection methods. Hydrostatic pressure maintains the sample composition during the sample loading, allowing the injection to be free of injection bias.
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U2 - 10.1021/ac0605142
DO - 10.1021/ac0605142
M3 - Article
C2 - 16944886
AN - SCOPUS:33748510386
SN - 0003-2700
VL - 78
SP - 6074
EP - 6080
JO - Analytical chemistry
JF - Analytical chemistry
IS - 17
ER -