TY - JOUR
T1 - Double recombination of a single immunoglobulin κ-chain allele
T2 - Implications for the mechanism of rearrangement
AU - Feddersen, R. M.
AU - Van Ness, B. G.
PY - 1985
Y1 - 1985
N2 - DNA fragments containing immunoglobulin κ-chain sequences from two different plasmacytomas (PC 3609 and PC 7043) were found by blot-hybridization studies to be dissociated from germ-line sequences on both the 3' and 5' ends. These fragments were cloned, sequenced, and found to contain the structural features of a product of two recombination events. Each contained a variable (V(κ)) gene segment recombined with a joining (J(κ)) gene segment followed by the characteristic κ light chain V-J reciprocal structure, a 5' J(κ) flanking sequence joined to a 3' V(κ) flanking sequence. These segments of DNA represent double recombination products (DRPs) of the same κ-chain allele. The DRP from PC 3609 contains a normal V-J1 recombination, while the DRP from PC 7043 contains an aberrant V-J2 recombination, resulting in a frameshift. The reciprocal structure in the PC 3609 DRP is the result of a V-J2 recombination; the reciprocal structure in the DRP of PC 7043 is the result of a V-J3 recombination and appears to have been derived directly from the productive κ-chain gene recombination in that plasmacytoma. These products demonstrate the capacity of a single κ light chain immunoglobulin allele to undergo multiple V-J recombinations. Furthermore, the presence of a V-J recombination and its reciprocal product in the same cell is inconsistent with a segregating mechanism, such as sister chromatid exchange, but is consistent with an inversion mechanism.
AB - DNA fragments containing immunoglobulin κ-chain sequences from two different plasmacytomas (PC 3609 and PC 7043) were found by blot-hybridization studies to be dissociated from germ-line sequences on both the 3' and 5' ends. These fragments were cloned, sequenced, and found to contain the structural features of a product of two recombination events. Each contained a variable (V(κ)) gene segment recombined with a joining (J(κ)) gene segment followed by the characteristic κ light chain V-J reciprocal structure, a 5' J(κ) flanking sequence joined to a 3' V(κ) flanking sequence. These segments of DNA represent double recombination products (DRPs) of the same κ-chain allele. The DRP from PC 3609 contains a normal V-J1 recombination, while the DRP from PC 7043 contains an aberrant V-J2 recombination, resulting in a frameshift. The reciprocal structure in the PC 3609 DRP is the result of a V-J2 recombination; the reciprocal structure in the DRP of PC 7043 is the result of a V-J3 recombination and appears to have been derived directly from the productive κ-chain gene recombination in that plasmacytoma. These products demonstrate the capacity of a single κ light chain immunoglobulin allele to undergo multiple V-J recombinations. Furthermore, the presence of a V-J recombination and its reciprocal product in the same cell is inconsistent with a segregating mechanism, such as sister chromatid exchange, but is consistent with an inversion mechanism.
UR - http://www.scopus.com/inward/record.url?scp=0022375583&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022375583&partnerID=8YFLogxK
U2 - 10.1073/pnas.82.14.4793
DO - 10.1073/pnas.82.14.4793
M3 - Article
C2 - 2991895
AN - SCOPUS:0022375583
SN - 0027-8424
VL - 82
SP - 4793
EP - 4797
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 14
ER -