Down-regulation of protein kinase C: A potential mechanism for 2-amino-3-methylimidazo[4,5-f]quinoline-mediated immunosuppression

Yong Woo Lee, Seung Hyun Han, Jae Hong Suh, Young Jin Jeon, Kyu Hwan Yang

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Immunosuppressive mechanism of food-born mutagenic and carcinogenic heterocyclic amine, 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), was studied in murine spleen cells. IQ suppressed the IL-2 secretion and its mRNA expression. Treatment of IQ also decreased PKC activity in both the membrane fraction and the cytosol fraction. Treatment of PMA resulted in the recovery of IQ-induced suppression of IL-2 production, but the addition of ionomycin (Io) had no effect. Subsequently, we examined the effect of IQ on the activation of NF-κB, AP-1, and NF-AT, which are key transcription factors for IL-2 transcription. The activation of NF-κB and AP-1 was markedly inhibited by IQ in PMA/PHA-stimulated cells. Meanwhile, NF-AT was not affected by IQ in PMA/Io-stimulated cells. These results suggest that the immunosuppression induced by IQ might be associated with the down-regulation of PKC and subsequent blockade of the activation of NF-κB and AP-1 in the IL-2 gene expression. Copyright (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

Original languageEnglish (US)
Pages (from-to)79-83
Number of pages5
JournalToxicology Letters
Volume102-103
DOIs
StatePublished - Dec 28 1998

Bibliographical note

Funding Information:
This work was supported by the Ministry of Science and Technology grant, ROK.

Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.

Keywords

  • 2-Amino-3-methylimidazo[4,5-f]quinoline
  • Immunosuppression
  • Protein kinase C

Fingerprint Dive into the research topics of 'Down-regulation of protein kinase C: A potential mechanism for 2-amino-3-methylimidazo[4,5-f]quinoline-mediated immunosuppression'. Together they form a unique fingerprint.

Cite this