Doxorubicin-induced mitochondrial dysfunction is secondary to nuclear p53 activation in H9c2 cardiomyoblasts

Vilma A. Sardão, Paulo J. Oliveira, Jon Holy, Catarina R. Oliveira, Kendall B. Wallace

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

Purpose: Doxorubicin (DOX) is a widely prescribed chemotherapeutic. The hypothesis for the present study is that DOX-induced myocyte apoptosis involves mitochondrial dysfunction that is a consequence of nuclear DOX effects. Methods: H9c2 myoblasts were incubated with 0, 0.5 and 1 μM DOX and nuclear and mitochondrial alterations were determined. Results: Doxorubicin accumulation in the nucleus was detected after 3 h treatment, followed by an increase in p53 and a decrease in mitochondrial membrane potential. Apoptotic markers, such as caspase activation and chromatin condensation were detected after 24 h of DOX treatment. Bax and p53 translocation to mitochondria as well as the formation of Bax clusters in the cytosol were observed. Importantly, pifithrin-alpha, a p53 inhibitor, protected against DOX-induced mitochondrial depolarization, caspase activation and cell death. Conclusion: Mitochondrial dysfunction in H9c2 myoblasts treated with DOX is a consequence of nuclear p53 activation rather than a direct effect of the drug on mitochondria.

Original languageEnglish (US)
Pages (from-to)811-827
Number of pages17
JournalCancer chemotherapy and pharmacology
Volume64
Issue number4
DOIs
StatePublished - Sep 2009

Bibliographical note

Funding Information:
Acknowledgments This work was supported by NIH grant HL 58016 and FCT SAU-OSM-64084-2006. Vilma A. Sardão is supported by grants from the Portuguese Foundation for Science and Technology, SFRH/BD/10251/2002 and SFRH/BPD/31549/2006. We acknowledge Ana Filipa Branco for excellent technical assistance. We also acknowledge Dr. Marc Fariss (University of Colorado, Denver) for providing vitamin E-succinate.

Keywords

  • Bax
  • Cardiomyopathy
  • Doxorubicin
  • H9c2 myoblast
  • Oxidative stress
  • P53

Fingerprint Dive into the research topics of 'Doxorubicin-induced mitochondrial dysfunction is secondary to nuclear p53 activation in H9c2 cardiomyoblasts'. Together they form a unique fingerprint.

Cite this