TY - JOUR
T1 - Dynamic localization of the nuclear import receptor and its interactions with transport factors
AU - Koepp, Deanna M.
AU - Wong, Darren H.
AU - Corbett, Anita H.
AU - Silver, Pamela A.
PY - 1996
Y1 - 1996
N2 - Characterization of the interactions between soluble factors required for nuclear transport is key to understanding the process of nuclear trafficking. Using a synthetic lethal screen with the rnal-1 strain, we have identified a genetic interaction between Rna1p, a GTPase activating protein required for nuclear transport, and yeast importin-β, a component of the nuclear localization signal receptor. By the use of fusion proteins, we demonstrate that Rna1p physically interacts with importin-β. Mutants in importin-β exhibit in vivo nuclear protein import defects, and importin-β localizes to the nuclear envelope along with other proteins associated with the nuclear pore complex. In addition, we present evidence that importin-α, but not importin-β, mislocalizes to the nucleus in cells where the GTPase Ran is likely to be in the GDP-bound state. We suggest a model of nuclear transport in which Ran-mediated hydrolysis of GTP is necessary for the import of importin-α and the nuclear localization signal-bearing substrate into the nucleus, while exchange of GDP for GTP on Ran is required for the export of both mRNA and importin-α from the nucleus.
AB - Characterization of the interactions between soluble factors required for nuclear transport is key to understanding the process of nuclear trafficking. Using a synthetic lethal screen with the rnal-1 strain, we have identified a genetic interaction between Rna1p, a GTPase activating protein required for nuclear transport, and yeast importin-β, a component of the nuclear localization signal receptor. By the use of fusion proteins, we demonstrate that Rna1p physically interacts with importin-β. Mutants in importin-β exhibit in vivo nuclear protein import defects, and importin-β localizes to the nuclear envelope along with other proteins associated with the nuclear pore complex. In addition, we present evidence that importin-α, but not importin-β, mislocalizes to the nucleus in cells where the GTPase Ran is likely to be in the GDP-bound state. We suggest a model of nuclear transport in which Ran-mediated hydrolysis of GTP is necessary for the import of importin-α and the nuclear localization signal-bearing substrate into the nucleus, while exchange of GDP for GTP on Ran is required for the export of both mRNA and importin-α from the nucleus.
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U2 - 10.1083/jcb.133.6.1163
DO - 10.1083/jcb.133.6.1163
M3 - Article
C2 - 8682856
AN - SCOPUS:0029897642
SN - 0021-9525
VL - 133
SP - 1163
EP - 1176
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 6
ER -