Early and late replicative chromosomal banding patterns of Gallus domesticus

Federico A Ponce de León, Y. Li, Z. Weng

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31 Scopus citations

Abstract

Early and late replicating chromosomal banding patterns of Gallus domesticus were investigated by cell synchronization and incorporation of 5'-bromodeoxyuridine during early and late DNA synthesis. The early replicating chromosomal banding patterns observed, as revealed by either acridine orange or Hoechst 33258/propidium iodide staining, were similar to the structural G-banding patterns obtained by trypsin di-gestion and Giemsa staining. Late replicating chromosomal banding showed extensive reverse band complementarity to the G-banding pattern. Cell synchronization increased the number of prometaphase and metaphase plates available for analysis. G-banding obtained by Hoechst 33258/propidium iodide staining was investigated due to the fact that it is compatible with chromosomal in situ hybridization procedures that use nonisotopically-labeled DNA probes. Standard replicative G-banded and R-banded idiograms, as obtained after cell synchronization, are proposed.

Original languageEnglish (US)
Pages (from-to)36-42
Number of pages7
JournalJournal of Heredity
Volume83
Issue number1
DOIs
StatePublished - Jan 1992

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