Early Detection of Foot-And-Mouth Disease Virus from Infected Cattle Using A Dry Filter Air Sampling System

J. M. Pacheco, B. Brito, E. Hartwig, G. R. Smoliga, A. Perez, J. Arzt, L. L. Rodriguez

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Foot-and-mouth disease (FMD) is a highly contagious livestock disease of high economic impact. Early detection of FMD virus (FMDV) is fundamental for rapid outbreak control. Air sampling collection has been demonstrated as a useful technique for detection of FMDV RNA in infected animals, related to the aerogenous nature of the virus. In the current study, air from rooms housing individual (n = 17) or two groups (n = 4) of cattle experimentally infected with FDMV A24 Cruzeiro of different virulence levels was sampled to assess the feasibility of applying air sampling as a non-invasive, screening tool to identify sources of FMDV infection. Detection of FMDV RNA in air was compared with first detection of clinical signs and FMDV RNA levels in serum and oral fluid. FMDV RNA was detected in room air samples 1–3 days prior (seven animals) or on the same day (four animals) as the appearance of clinical signs in 11 of 12 individually housed cattle. Only in one case clinical signs preceded detection in air samples by one day. Overall, viral RNA in oral fluid or serum preceded detection in air samples by 1–2 days. Six individually housed animals inoculated with attenuated strains did not show clinical signs, but virus was detected in air in one of these cases 3 days prior to first detection in oral fluid. In groups of four cattle housed together, air detection always preceded appearance of clinical signs by 1–2 days and coincided more often with viral shedding in oral fluid than virus in blood. These data confirm that air sampling is an effective non-invasive screening method for detecting FMDV infection in confined to enclosed spaces (e.g. auction barns, milking parlours). This technology could be a useful tool as part of a surveillance strategy during FMD prevention, control or eradication efforts.

Original languageEnglish (US)
Pages (from-to)564-573
Number of pages10
JournalTransboundary and Emerging Diseases
Volume64
Issue number2
DOIs
StatePublished - Apr 1 2017

Bibliographical note

Funding Information:
This research was funded primarily by ARS-CRIS project 8064-32000-057-00D. BB was recipient of Plum Island Animal Disease Center Research Participation Program fellowships, administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement with the US Department of Energy. The authors wish to acknowledge Drs. Tam Garland, John Vitko, Elizabeth George from the Department of Homeland Security and Michael Farrell from the Department of Defense for providing air sampling equipment. We also thank the multiple researchers and projects at PIADC for providing access to air collection during their experiments.

Publisher Copyright:
Published 2015. This article is a U.S. Government work and is in the public domain in the USA.

Keywords

  • air samplers
  • airborne
  • dry filter unit
  • foot-and-mouth disease
  • foot-and-mouth disease virus
  • spread

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