Effect of 6-hydroxydopamine on the incorporation of 14C-leucine into rat brain protein

Horace H. Loh; S. Stolman; C. Y. Lee

doi:10.1016/0024-3205(71)90277-3

Effect of 6-hydroxydopamine on the incorporation of ¹⁴C-leucine into rat brain protein

Horace H. Loh, S. Stolman, C. Y. Lee

Pharmacology

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Abstract

The effect of 6-hydroxydopamine on in vitro and in vivo cerebral protein synthesis was studied in rats. Male rats receiving 200 μg of 6-hydroxydopamine intracisternally daily for three injections showed a significant decrease in incorporating activity of ¹⁴C-leucine into brain microsomal and synaptosomal proteins over that of control animals in vitro. Experiments with intracisternally injected ¹⁴C-leucine also showed a decreased incorporation of labeled amino acid in 6-hydroxydopamine treated animals in mitochondria, crude nuclei, "noncholinergic" synaptosome and myelin fraction, while no change was demonstrated in the other fractions. The degeneration of adrenergic nerve endings by 6-hydroxydopamine may be related to its inhibitory action on brain protein synthesis.

Original language	English (US)
Pages (from-to)	1171-1180
Number of pages	10
Journal	Life Sciences
Volume	10
Issue number	20 PART 1
DOIs	https://doi.org/10.1016/0024-3205(71)90277-3
State	Published - Oct 15 1971

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title = "Effect of 6-hydroxydopamine on the incorporation of 14C-leucine into rat brain protein",

abstract = "The effect of 6-hydroxydopamine on in vitro and in vivo cerebral protein synthesis was studied in rats. Male rats receiving 200 μg of 6-hydroxydopamine intracisternally daily for three injections showed a significant decrease in incorporating activity of 14C-leucine into brain microsomal and synaptosomal proteins over that of control animals in vitro. Experiments with intracisternally injected 14C-leucine also showed a decreased incorporation of labeled amino acid in 6-hydroxydopamine treated animals in mitochondria, crude nuclei, {"}noncholinergic{"} synaptosome and myelin fraction, while no change was demonstrated in the other fractions. The degeneration of adrenergic nerve endings by 6-hydroxydopamine may be related to its inhibitory action on brain protein synthesis.",

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AU - Loh, Horace H.

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AB - The effect of 6-hydroxydopamine on in vitro and in vivo cerebral protein synthesis was studied in rats. Male rats receiving 200 μg of 6-hydroxydopamine intracisternally daily for three injections showed a significant decrease in incorporating activity of 14C-leucine into brain microsomal and synaptosomal proteins over that of control animals in vitro. Experiments with intracisternally injected 14C-leucine also showed a decreased incorporation of labeled amino acid in 6-hydroxydopamine treated animals in mitochondria, crude nuclei, "noncholinergic" synaptosome and myelin fraction, while no change was demonstrated in the other fractions. The degeneration of adrenergic nerve endings by 6-hydroxydopamine may be related to its inhibitory action on brain protein synthesis.

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Effect of 6-hydroxydopamine on the incorporation of ¹⁴C-leucine into rat brain protein

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