Effect of aging on the interaction of quinuclidinyl benzilate, N-methylscopolamine, pirenzepine, and gallamine with brain muscarinic receptors

Wanida Surichamorn; Ok Nyu Kim; Norman H. Lee; Wi S. Lai; Esam E. El-Fakahany

doi:10.1007/BF00971637

Effect of aging on the interaction of quinuclidinyl benzilate, N-methylscopolamine, pirenzepine, and gallamine with brain muscarinic receptors

Wanida Surichamorn, Ok Nyu Kim, Norman H. Lee, Wi S. Lai, Esam E. El-Fakahany

Pharmacy Research and Graduate Education

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Abstract

The objective of the present study was to investigate the effects of senescence on the binding characteristics of muscarinic receptors by using [³H]quinuclidinyl benzilate ([³H]QNB) and [³H]N-methylscopolamine ([³H]NMS) as ligands in young (3months), middle-age (10months) and old (24 months) male Fischer 344 rats. Muscarinic receptor density was found to decrease significantly with aging in certain brain regions, depending on the ligand employed. Moreover, the relative proportions of M₁ and M₂ muscarinic receptor subtypes was not significantly altered by aging, except in the aged striatum. Furthermore, the dissociation kinetics of [³H]NMS in the cerebral cortex and their allosteric modulation by gallamine were only slightly influenced by age.

Original language	English (US)
Pages (from-to)	1183-1191
Number of pages	9
Journal	Neurochemical Research
Volume	13
Issue number	12
DOIs	https://doi.org/10.1007/BF00971637
State	Published - Dec 1988

Keywords

Muscarinic receptors
aging
allosteric
brain
gallamine
pirenzepine
subtypes

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title = "Effect of aging on the interaction of quinuclidinyl benzilate, N-methylscopolamine, pirenzepine, and gallamine with brain muscarinic receptors",

abstract = "The objective of the present study was to investigate the effects of senescence on the binding characteristics of muscarinic receptors by using [3H]quinuclidinyl benzilate ([3H]QNB) and [3H]N-methylscopolamine ([3H]NMS) as ligands in young (3months), middle-age (10months) and old (24 months) male Fischer 344 rats. Muscarinic receptor density was found to decrease significantly with aging in certain brain regions, depending on the ligand employed. Moreover, the relative proportions of M1 and M2 muscarinic receptor subtypes was not significantly altered by aging, except in the aged striatum. Furthermore, the dissociation kinetics of [3H]NMS in the cerebral cortex and their allosteric modulation by gallamine were only slightly influenced by age.",

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AU - Lee, Norman H.

AU - Lai, Wi S.

AU - El-Fakahany, Esam E.

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Effect of aging on the interaction of quinuclidinyl benzilate, N-methylscopolamine, pirenzepine, and gallamine with brain muscarinic receptors

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