Effect of carbohydrate on protein solubility

Erlinda Q. Lawson; B. O.E. Hedlund; Marna E. Ericson; Deborah A. Mood; Gary W. Litman; Russell Middaugh

doi:10.1016/0003-9861(83)90449-6

Effect of carbohydrate on protein solubility

Erlinda Q. Lawson, B. O.E. Hedlund, Marna E. Ericson, Deborah A. Mood, Gary W. Litman, Russell Middaugh

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Abstract

The effect of covalently attached carbohydrate on the solubility of a number of proteins has been examined by the PEG precipitation technique. Both increases and decreases in solubility are observed depending on the state of glycosylation, the type of protein, and temperature. It is concluded from this data and associated apparent thermodynamic parameters that a general role for carbohydrate in the solubilization of proteins is not currently experimentally supportable.

Original language	English (US)
Pages (from-to)	572-575
Number of pages	4
Journal	Archives of Biochemistry and Biophysics
Volume	220
Issue number	2
DOIs	https://doi.org/10.1016/0003-9861(83)90449-6
State	Published - Feb 1 1983

Bibliographical note

Funding Information:
This work was supported by the National Science Foundation (PCM 8011193) and the National Institutes of Health (AI 13528 and AM 28124). E.Q.L. is supported by a fellowship from the Cancer Research Institute.

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title = "Effect of carbohydrate on protein solubility",

abstract = "The effect of covalently attached carbohydrate on the solubility of a number of proteins has been examined by the PEG precipitation technique. Both increases and decreases in solubility are observed depending on the state of glycosylation, the type of protein, and temperature. It is concluded from this data and associated apparent thermodynamic parameters that a general role for carbohydrate in the solubilization of proteins is not currently experimentally supportable.",

author = "Lawson, {Erlinda Q.} and Hedlund, {B. O.E.} and Ericson, {Marna E.} and Mood, {Deborah A.} and Litman, {Gary W.} and Russell Middaugh",

note = "Funding Information: This work was supported by the National Science Foundation (PCM 8011193) and the National Institutes of Health (AI 13528 and AM 28124). E.Q.L. is supported by a fellowship from the Cancer Research Institute.",

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T1 - Effect of carbohydrate on protein solubility

AU - Lawson, Erlinda Q.

AU - Hedlund, B. O.E.

AU - Ericson, Marna E.

AU - Mood, Deborah A.

AU - Litman, Gary W.

AU - Middaugh, Russell

N1 - Funding Information: This work was supported by the National Science Foundation (PCM 8011193) and the National Institutes of Health (AI 13528 and AM 28124). E.Q.L. is supported by a fellowship from the Cancer Research Institute.

PY - 1983/2/1

Y1 - 1983/2/1

N2 - The effect of covalently attached carbohydrate on the solubility of a number of proteins has been examined by the PEG precipitation technique. Both increases and decreases in solubility are observed depending on the state of glycosylation, the type of protein, and temperature. It is concluded from this data and associated apparent thermodynamic parameters that a general role for carbohydrate in the solubilization of proteins is not currently experimentally supportable.

AB - The effect of covalently attached carbohydrate on the solubility of a number of proteins has been examined by the PEG precipitation technique. Both increases and decreases in solubility are observed depending on the state of glycosylation, the type of protein, and temperature. It is concluded from this data and associated apparent thermodynamic parameters that a general role for carbohydrate in the solubilization of proteins is not currently experimentally supportable.

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JO - Archives of Biochemistry and Biophysics

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Effect of carbohydrate on protein solubility

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