Effect of chlortetracycline on the growth and intracellular components of Spirulina platensis and its biodegradation pathway

Ting Zhou; Leipeng Cao; Qi Zhang; Yuhuan Liu; Shuyu Xiang; Tongying Liu; Roger Ruan

doi:10.1016/j.jhazmat.2021.125310

Effect of chlortetracycline on the growth and intracellular components of Spirulina platensis and its biodegradation pathway

Ting Zhou, Leipeng Cao, Qi Zhang, Yuhuan Liu, Shuyu Xiang, Tongying Liu, Roger Ruan

Bioproducts and Biosystems Engineering

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Chlortetracycline (CTC) usually presents in livestock wastewater with oxytetracycline (OTC), causing damage to human health and ecosystems. It's urgent to seek low-cost and ecofriendly technology for antibiotics removal. In this study, effects of CTC and CTC + OTC (CTC:OTC= 1:1, g/g) on Spirulina platensis have been investigated. EC₅₀ value of CTC for S. platensis was 8.76 mg/L at 96 h and risk quotient value in wastewater was 15.85. Inhibition of CTC on S. platensis gradually enhanced with increase of CTC, but CTC + OTC below 1.0 mg/L didn't harm the growth of microalgae. Chlorophyll-a (Chl-a) and carotenoid content showed a parabolic trend with extension of time at CTC group. Chl-a synthesis gradually decreased with increase of CTC + OTC stress. High concentrations of CTC and CTC + OTC showed obvious inhibition on phycocyanin production. Polyunsaturated fatty acids (PUFA) and saturated fatty acids (SFA) contents peaked at 1.0 mg/L CTC, corresponding to the minimum of superoxide dismutase (SOD) activity in S. platensis. SFA and PUFA contents decreased when CTC + OTC content was above 2.0 mg/L. CTC and CTC + OTC (both over 2.0 mg/L) stimulated production of dissolved extracellular organic matters in S. platensis. Removal efficiency of CTC by S. platensis was about 98.63–99.95% and its biodegradation pathways were hydroxylation and side-chain breakdown.

Original language	English (US)
Article number	125310
Journal	Journal of Hazardous Materials
Volume	413
DOIs	https://doi.org/10.1016/j.jhazmat.2021.125310
State	Published - Jul 5 2021

Bibliographical note

Funding Information:
This work was supported by Research Project of State Key Laboratory of Food Science and Technology, Nanchang University ( SKLF-ZZB-201915 ); The Key Project of Jiangxi Provincial Department of Science and Technology ( 20181BAB206030 , 20181BBF60026 , GCXZ2014-124 , 20161BBF60057 ); National Natural Science Foundation of China (No. 21878139 , 21878237 , 21706087 , 21466022 , 21766019 , 51708308 ) and the Post-doctoral Innovative Talents Support Program of China ( BX20190147 ).

Publisher Copyright:
© 2021 Elsevier B.V.

Keywords

Biodegradation pathway
CTC
Dissolved extracellular organic matters
Intracellular metabolites
S. platensis

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Effect of chlortetracycline on the growth and intracellular components of Spirulina platensis and its biodegradation pathway",

abstract = "Chlortetracycline (CTC) usually presents in livestock wastewater with oxytetracycline (OTC), causing damage to human health and ecosystems. It's urgent to seek low-cost and ecofriendly technology for antibiotics removal. In this study, effects of CTC and CTC + OTC (CTC:OTC= 1:1, g/g) on Spirulina platensis have been investigated. EC50 value of CTC for S. platensis was 8.76 mg/L at 96 h and risk quotient value in wastewater was 15.85. Inhibition of CTC on S. platensis gradually enhanced with increase of CTC, but CTC + OTC below 1.0 mg/L didn't harm the growth of microalgae. Chlorophyll-a (Chl-a) and carotenoid content showed a parabolic trend with extension of time at CTC group. Chl-a synthesis gradually decreased with increase of CTC + OTC stress. High concentrations of CTC and CTC + OTC showed obvious inhibition on phycocyanin production. Polyunsaturated fatty acids (PUFA) and saturated fatty acids (SFA) contents peaked at 1.0 mg/L CTC, corresponding to the minimum of superoxide dismutase (SOD) activity in S. platensis. SFA and PUFA contents decreased when CTC + OTC content was above 2.0 mg/L. CTC and CTC + OTC (both over 2.0 mg/L) stimulated production of dissolved extracellular organic matters in S. platensis. Removal efficiency of CTC by S. platensis was about 98.63–99.95% and its biodegradation pathways were hydroxylation and side-chain breakdown.",

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author = "Ting Zhou and Leipeng Cao and Qi Zhang and Yuhuan Liu and Shuyu Xiang and Tongying Liu and Roger Ruan",

note = "Funding Information: This work was supported by Research Project of State Key Laboratory of Food Science and Technology, Nanchang University ( SKLF-ZZB-201915 ); The Key Project of Jiangxi Provincial Department of Science and Technology ( 20181BAB206030 , 20181BBF60026 , GCXZ2014-124 , 20161BBF60057 ); National Natural Science Foundation of China (No. 21878139 , 21878237 , 21706087 , 21466022 , 21766019 , 51708308 ) and the Post-doctoral Innovative Talents Support Program of China ( BX20190147 ). Publisher Copyright: {\textcopyright} 2021 Elsevier B.V.",

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AU - Liu, Yuhuan

AU - Xiang, Shuyu

AU - Liu, Tongying

AU - Ruan, Roger

N1 - Funding Information: This work was supported by Research Project of State Key Laboratory of Food Science and Technology, Nanchang University ( SKLF-ZZB-201915 ); The Key Project of Jiangxi Provincial Department of Science and Technology ( 20181BAB206030 , 20181BBF60026 , GCXZ2014-124 , 20161BBF60057 ); National Natural Science Foundation of China (No. 21878139 , 21878237 , 21706087 , 21466022 , 21766019 , 51708308 ) and the Post-doctoral Innovative Talents Support Program of China ( BX20190147 ). Publisher Copyright: © 2021 Elsevier B.V.

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N2 - Chlortetracycline (CTC) usually presents in livestock wastewater with oxytetracycline (OTC), causing damage to human health and ecosystems. It's urgent to seek low-cost and ecofriendly technology for antibiotics removal. In this study, effects of CTC and CTC + OTC (CTC:OTC= 1:1, g/g) on Spirulina platensis have been investigated. EC50 value of CTC for S. platensis was 8.76 mg/L at 96 h and risk quotient value in wastewater was 15.85. Inhibition of CTC on S. platensis gradually enhanced with increase of CTC, but CTC + OTC below 1.0 mg/L didn't harm the growth of microalgae. Chlorophyll-a (Chl-a) and carotenoid content showed a parabolic trend with extension of time at CTC group. Chl-a synthesis gradually decreased with increase of CTC + OTC stress. High concentrations of CTC and CTC + OTC showed obvious inhibition on phycocyanin production. Polyunsaturated fatty acids (PUFA) and saturated fatty acids (SFA) contents peaked at 1.0 mg/L CTC, corresponding to the minimum of superoxide dismutase (SOD) activity in S. platensis. SFA and PUFA contents decreased when CTC + OTC content was above 2.0 mg/L. CTC and CTC + OTC (both over 2.0 mg/L) stimulated production of dissolved extracellular organic matters in S. platensis. Removal efficiency of CTC by S. platensis was about 98.63–99.95% and its biodegradation pathways were hydroxylation and side-chain breakdown.

AB - Chlortetracycline (CTC) usually presents in livestock wastewater with oxytetracycline (OTC), causing damage to human health and ecosystems. It's urgent to seek low-cost and ecofriendly technology for antibiotics removal. In this study, effects of CTC and CTC + OTC (CTC:OTC= 1:1, g/g) on Spirulina platensis have been investigated. EC50 value of CTC for S. platensis was 8.76 mg/L at 96 h and risk quotient value in wastewater was 15.85. Inhibition of CTC on S. platensis gradually enhanced with increase of CTC, but CTC + OTC below 1.0 mg/L didn't harm the growth of microalgae. Chlorophyll-a (Chl-a) and carotenoid content showed a parabolic trend with extension of time at CTC group. Chl-a synthesis gradually decreased with increase of CTC + OTC stress. High concentrations of CTC and CTC + OTC showed obvious inhibition on phycocyanin production. Polyunsaturated fatty acids (PUFA) and saturated fatty acids (SFA) contents peaked at 1.0 mg/L CTC, corresponding to the minimum of superoxide dismutase (SOD) activity in S. platensis. SFA and PUFA contents decreased when CTC + OTC content was above 2.0 mg/L. CTC and CTC + OTC (both over 2.0 mg/L) stimulated production of dissolved extracellular organic matters in S. platensis. Removal efficiency of CTC by S. platensis was about 98.63–99.95% and its biodegradation pathways were hydroxylation and side-chain breakdown.

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KW - Intracellular metabolites

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ER -

Effect of chlortetracycline on the growth and intracellular components of Spirulina platensis and its biodegradation pathway

Abstract

Bibliographical note

Keywords

UN SDGs

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