Effect of ferric nitrilotriacetate on predominantly cortical neuronal cell cultures

Kenneth F Swaiman; Valynda L. Machen

doi:10.1007/BF00964879

Effect of ferric nitrilotriacetate on predominantly cortical neuronal cell cultures

Kenneth F Swaiman, Valynda L. Machen

Neurology

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7 Scopus citations

Abstract

Predominately neuronal cell cultures were produced as described in previous communications. Neuronal cells were exposed to ferric nitrilotriacetate (Fe-NTA) at varying concentrations. Studies of the neuronal cells were performed at 13 and 20 days in culture. In addition to morphologic studies, biochemical assays including choline acetyltransferase (ChAT) activity, specific [³H]flunitrazepam (FLU) binding, clonazepam (CLO)-displaceable [³H]FLU binding, Ro5-4864-displaceable [³H]FLU binding, high-affinity [³H]GABA uptake, and protein determinations were performed. The data demonstrate that chelated ferric iron has an adverse effect on predominately neuronal cultures after 7 days of exposure as measured by choline acetyltransferase activity, while other measures remained unaffected; however, after 14 days of exposure all measures were significantly decreased. The effects of Fe-NTA exposure appear to be both concentration and duration-of-exposure related.

Original language	English (US)
Pages (from-to)	683-688
Number of pages	6
Journal	Neurochemical Research
Volume	14
Issue number	7
DOIs	https://doi.org/10.1007/BF00964879
State	Published - Jul 1 1989

Keywords

Hallervorden-Spatz syndrome
Neuronal cultures
Parkinson disease
benzodiazepine
clonazepam
iron toxicity

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title = "Effect of ferric nitrilotriacetate on predominantly cortical neuronal cell cultures",

abstract = "Predominately neuronal cell cultures were produced as described in previous communications. Neuronal cells were exposed to ferric nitrilotriacetate (Fe-NTA) at varying concentrations. Studies of the neuronal cells were performed at 13 and 20 days in culture. In addition to morphologic studies, biochemical assays including choline acetyltransferase (ChAT) activity, specific [3H]flunitrazepam (FLU) binding, clonazepam (CLO)-displaceable [3H]FLU binding, Ro5-4864-displaceable [3H]FLU binding, high-affinity [3H]GABA uptake, and protein determinations were performed. The data demonstrate that chelated ferric iron has an adverse effect on predominately neuronal cultures after 7 days of exposure as measured by choline acetyltransferase activity, while other measures remained unaffected; however, after 14 days of exposure all measures were significantly decreased. The effects of Fe-NTA exposure appear to be both concentration and duration-of-exposure related.",

keywords = "Hallervorden-Spatz syndrome, Neuronal cultures, Parkinson disease, benzodiazepine, clonazepam, iron toxicity",

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AU - Swaiman, Kenneth F

AU - Machen, Valynda L.

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N2 - Predominately neuronal cell cultures were produced as described in previous communications. Neuronal cells were exposed to ferric nitrilotriacetate (Fe-NTA) at varying concentrations. Studies of the neuronal cells were performed at 13 and 20 days in culture. In addition to morphologic studies, biochemical assays including choline acetyltransferase (ChAT) activity, specific [3H]flunitrazepam (FLU) binding, clonazepam (CLO)-displaceable [3H]FLU binding, Ro5-4864-displaceable [3H]FLU binding, high-affinity [3H]GABA uptake, and protein determinations were performed. The data demonstrate that chelated ferric iron has an adverse effect on predominately neuronal cultures after 7 days of exposure as measured by choline acetyltransferase activity, while other measures remained unaffected; however, after 14 days of exposure all measures were significantly decreased. The effects of Fe-NTA exposure appear to be both concentration and duration-of-exposure related.

AB - Predominately neuronal cell cultures were produced as described in previous communications. Neuronal cells were exposed to ferric nitrilotriacetate (Fe-NTA) at varying concentrations. Studies of the neuronal cells were performed at 13 and 20 days in culture. In addition to morphologic studies, biochemical assays including choline acetyltransferase (ChAT) activity, specific [3H]flunitrazepam (FLU) binding, clonazepam (CLO)-displaceable [3H]FLU binding, Ro5-4864-displaceable [3H]FLU binding, high-affinity [3H]GABA uptake, and protein determinations were performed. The data demonstrate that chelated ferric iron has an adverse effect on predominately neuronal cultures after 7 days of exposure as measured by choline acetyltransferase activity, while other measures remained unaffected; however, after 14 days of exposure all measures were significantly decreased. The effects of Fe-NTA exposure appear to be both concentration and duration-of-exposure related.

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KW - Neuronal cultures

KW - Parkinson disease

KW - benzodiazepine

KW - clonazepam

KW - iron toxicity

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Effect of ferric nitrilotriacetate on predominantly cortical neuronal cell cultures

Abstract

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