Effect of ferric nitrilotriacetate on predominantly cortical neuronal cell cultures

Kenneth F Swaiman, Valynda L. Machen

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7 Scopus citations

Abstract

Predominately neuronal cell cultures were produced as described in previous communications. Neuronal cells were exposed to ferric nitrilotriacetate (Fe-NTA) at varying concentrations. Studies of the neuronal cells were performed at 13 and 20 days in culture. In addition to morphologic studies, biochemical assays including choline acetyltransferase (ChAT) activity, specific [3H]flunitrazepam (FLU) binding, clonazepam (CLO)-displaceable [3H]FLU binding, Ro5-4864-displaceable [3H]FLU binding, high-affinity [3H]GABA uptake, and protein determinations were performed. The data demonstrate that chelated ferric iron has an adverse effect on predominately neuronal cultures after 7 days of exposure as measured by choline acetyltransferase activity, while other measures remained unaffected; however, after 14 days of exposure all measures were significantly decreased. The effects of Fe-NTA exposure appear to be both concentration and duration-of-exposure related.

Original languageEnglish (US)
Pages (from-to)683-688
Number of pages6
JournalNeurochemical Research
Volume14
Issue number7
DOIs
StatePublished - Jul 1 1989

Keywords

  • Hallervorden-Spatz syndrome
  • Neuronal cultures
  • Parkinson disease
  • benzodiazepine
  • clonazepam
  • iron toxicity

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