Effect of insulin on a serum‐free hybridoma culture

Weichang Zhou, Wei‐Shou ‐S Hu

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Insulin is often included in serum‐free media for animal cell cultivation. However, the necessity of insulin for a specific cell line is rather uncertain. In this article we report the effects of insulin on the cultivation of a hybridoma cell line in a serum‐free medium. It was found that insulin affected neither the cell growth nor the antibody production. The specific growth rate and specific antibody production rate were very similar in the cultures with or without insulin. However, the presence of insulin affected the nutrient consumption rate and cell metabolism. Including insulin in the medium resulted in a higher specific glucose consumption rate, a shorter exponential growth stage, and a lower final antibody concentration. The elimination of insulin from the medium allowed antibody to accumulate to a concentration substantially higher than that in the insulin‐containing cultuvre. © 1995 John Wiley & Sons, Inc.

Original languageEnglish (US)
Pages (from-to)181-185
Number of pages5
JournalBiotechnology and bioengineering
Issue number2
StatePublished - Jul 20 1995


  • antibody production
  • cell growth
  • glucose metabolism
  • insulin

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