Effect of Irrigants and Cementum Injury on Diffusion of Hydroxyl Ions through the Dentinal Tubules

Sherma Saif, Clifton M. Carey, Patricia A. Tordik, Scott B. McClanahan

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


This study measured hydroxyl ion diffusion through dentinal tubules into a bathing solution. Eighty single-canal, instrumented teeth were divided into 8 groups. Control groups 1 and 3 were irrigated with 10 mL 0.9% saline and 10 mL 6% sodium hypochlorite (NaOCl), respectively. Control groups 5 and 7 were irrigated with 3 mL and 1 mL 17% ethylenediaminetetraacetic acid (EDTA) and then 10 mL 6% NaOCl, respectively. Experimental groups 2, 4, 6, and 8 were irrigated as groups 1, 3, 5, and 7, followed by placement of calcium hydroxide (Ca(OH)2) into canals. Bathing solution pH was recorded for 30 days, a cementum defect was made, and then pH was recorded for another 30 days. With a paired difference test, average pH during steady state was statistically different and higher after the defect (P < .001). With Tukey multiple comparisons, post-defect pH for group 6 was found to be significantly greater (P < .01) than in other groups. This study indicated final canal irrigation with 3 mL 17% EDTA and 10 mL 6% NaOCl before Ca(OH)2 placement allowed the greatest hydroxyl ion diffusion to the root surface.

Original languageEnglish (US)
Pages (from-to)50-52
Number of pages3
JournalJournal of Endodontics
Issue number1
StatePublished - Jan 2008

Bibliographical note

Funding Information:
This study was supported by USPHS Research Grant DE 14707 to the American Dental Association Foundation from the National Institutes of Health – National Institute of Dental and Craniofacial Research and is part of the dental research program conducted by the National Institute of Standards and Technology in cooperation with the American Dental Association Foundation.

Copyright 2009 Elsevier B.V., All rights reserved.


  • Calcium hydroxide
  • diffusion
  • ethylenediaminetetraacetic acid
  • hydroxyl ions
  • smear layer


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