Effect of Irrigants and Cementum Injury on Diffusion of Hydroxyl Ions through the Dentinal Tubules

Sherma Saif, Clifton M. Carey, Patricia A. Tordik, Scott B. McClanahan

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


This study measured hydroxyl ion diffusion through dentinal tubules into a bathing solution. Eighty single-canal, instrumented teeth were divided into 8 groups. Control groups 1 and 3 were irrigated with 10 mL 0.9% saline and 10 mL 6% sodium hypochlorite (NaOCl), respectively. Control groups 5 and 7 were irrigated with 3 mL and 1 mL 17% ethylenediaminetetraacetic acid (EDTA) and then 10 mL 6% NaOCl, respectively. Experimental groups 2, 4, 6, and 8 were irrigated as groups 1, 3, 5, and 7, followed by placement of calcium hydroxide (Ca(OH)2) into canals. Bathing solution pH was recorded for 30 days, a cementum defect was made, and then pH was recorded for another 30 days. With a paired difference test, average pH during steady state was statistically different and higher after the defect (P < .001). With Tukey multiple comparisons, post-defect pH for group 6 was found to be significantly greater (P < .01) than in other groups. This study indicated final canal irrigation with 3 mL 17% EDTA and 10 mL 6% NaOCl before Ca(OH)2 placement allowed the greatest hydroxyl ion diffusion to the root surface.

Original languageEnglish (US)
Pages (from-to)50-52
Number of pages3
JournalJournal of Endodontics
Issue number1
StatePublished - Jan 1 2008


  • Calcium hydroxide
  • diffusion
  • ethylenediaminetetraacetic acid
  • hydroxyl ions
  • smear layer

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